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Research Article Open access CC BY 4.0

Effect of Fermentation Time of Bacillus subtilis ATCC 6051 on Black and Red Lima Bean (Phaseolus lunatus) Seeds on Nutrients, Antinutrients and Antioxidant Potential

Kouadio Patrick N’zi, Messou Tchumou, Koffi Siméon Brou, Kouadio Constant Attchelouwa, Kouadio Florent N’guessan, Kablan Tano

Biotechnology Journal International · pp. 151–164 · Published 11 Dec 2024

10.9734/bji/2024/v28i6758

Abstract

Aims: The lima bean (Phaseolus lunatus) is a species of legume rich in protein and minerals, particularly potassium, phosphorus, calcium and iron. However, this legume is not widely consumed because it is considered a poor man's protein and its consumption leads to digestion problems due to its high content of anti-nutritional factors. The aim of this study is therefore to add value to Phaseolus lunatus by improving its nutritional and antioxidant quality through controlled fermentation with Bacillus subtilis ATCC 6051 in order to make it a food ingredient. Study Design: Phaseolus lunatus seed flour was fermented with Bacillus subtilis ATCC 6051 for 24, 48 and 72 hours under controlled conditions. Methodology: The impact of fermentation time with Bacillus subtilis on the proximal composition, anti-nutritional factors and antioxidant capacity of lima bean flour was evaluated. Results: Protein and fibre contents increased during fermentation (19.77 to 22.93%) and (5.99 to 8.28%) while lipid and carbohydrate contents decreased. The levels of anti-nutritional factors decreased significantly (p < 0.05). Antioxidant power increased significantly (p < 0.05) during fermentation. Conclusion: Fermentation with Bacillus subtilis improves the nutritional quality and antioxidant power of lima bean flour. Lima bean flour fermented with Bacillus subtilis could be used as a human food ingredient.

Lima bean fermentation nutrient anti-nutrition factors antioxidant activity Bacillus sutilis

References (23)

  1. 1 Two new flavonoids and other constituents in licorice root. Their relative astringency and radical scavenging effects. [DOI]
  2. 2 Plant proteins in relation to human protein and amino acid nutrition [DOI]
  3. 3 Proanthocyanidins and hydrolysable tannins: occurrence, dietary intake and pharmacological effects [DOI]
  4. 4 Fluorescent probes and bioimaging: alkali metals, alkaline earth metals and pH [DOI]
  5. 5 Production of bioactive peptides during soybean fermentation and their potential health benefits [DOI]
  6. 6 Improved physicochemical and functional properties of dietary fiber from millet bran fermented by Bacillus natto [DOI]
  7. 7 Zinc and Copper Intakes and Their Major Food Sources for Older Adults in the 1994–96 Continuing Survey of Food Intakes by Individuals (CSFII) [DOI]
  8. 8 Traditional Fermented Foods and Beverages from a Microbiological and Nutritional Perspective: The Colombian Heritage [DOI]
  9. 9 A review phytic acid: As antinutrient or nutraceutical
  10. 10 A review of oxalate poisoning in domestic animals: tolerance and performance aspects [DOI]
  11. 11 Fermentation of cereals for specific purpose
  12. 12 Significance of Fermented Food in Nutrition and Food Science [DOI]
  13. 13 Disorders of Calcium: Hypercalcemia and Hypocalcemia [DOI]
  14. 14 Ultrasound-assisted liquid-state fermentation of soybean meal with Bacillus subtilis: Effects on peptides content, ACE inhibitory activity and biomass [DOI]
  15. 15 Performance and Histological Responses of Internal Organs of Broiler Chickens Fed Raw, Dehulled, and Aqueous and Dry-Heated Kidney Bean Meals [DOI]
  16. 16 The Effect of Soaking, Boiling and Fermentation with Rhizopus oligosporus on the Water Soluble Vitamin Content of Bambara Groundnut [DOI]
  17. 17 Antioxidant Properties and Total Phenolics of Thua Nao (a Thai Fermented Soybean)as Affected by Bacillus-fermentation [DOI]
  18. 18 Biological and chemical changes during the aerobic and anaerobic fermentation of African locust bean
  19. 19 Influence of solid state fermentation with Bacillus subtilis 67 strain on the nutritional value of rapeseed meal and its effects on performance and meat quality of broiler chickens [DOI]
  20. 20 Review of Copper Provision in the Parenteral Nutrition of Adults [DOI]
  21. 21 Effect of Bacillus sphaericus on Proximate Composition of Soybean (Glycine max) for the Production of Soy Iru [DOI]
  22. 22 Increased organic acid production and decreased phytate phosphorus by high level of water content of Bacillus subtilis ATCC PTA-6737 fermentation of soybean meal [DOI]
  23. 23 STARTER CULTURES AND THEIR ROLE IN FERMENTED FOODS [DOI]

Cited by 2

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AGNES VIRGINIA SIMAMORA, Lince Mukkun, Mayavira V. Hahuly · BIO Web of Conferences · 2026

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