Isolation, Identification and Antibiogram of Bacteria Isolated from Raw Cow Milk in Dutsin-Ma, Katsina State

Abstract

The study was conducted to isolate and identify the bacteria, to know the sources of contamination of milk and antibiotic sensitivity of bacteria obtained from Dutsin-Ma, Katsina State. A total of 45 samples were collected from different locations in Dutsin-Ma such as Wednesday market, opposite the FUDMA takeoff site and Hospital road. All these samples were analyzed by culturing in different media such as Salmonella-Shigella agar, Eosin Methylene Blue agar, Mannitol Salt Agar, Nutrient agar, Cetrimide agar, and MacConkey agar. Biochemical tests were performed to identify the organism. Among 45 samples, 20 (37.7%) were Staphylococcus spp. Similarly, 11 (20.8%), 6 (11.3%), 4(7.5%) and 12(22.6%) were found positive for Escherichia coli, Pseudomonas aeruginosa, Klebsiella and Salmonella spp. and. respectively. Results of the antibiotic sensitivity test represent that, out of ten antibiotics Staphylococcus sp. were very sensitive against Gentamicin (95%), Ciprofloxacin (90%), Streptomycin (70%), and highly resistant against Zinnacef (60%), Ampiclox (70%), Amoxicillin (50%). Salmonella sp. were highly sensitive to Pefloxacin (83.3%), Sparfloxacin (83.3%), and Ciprofloxacin (95%), but resistant against Augmentin (83.3%), Streptomycin (75%), Sulfamethoxazole (66.6%). Klebsiella spp. were highly sensitive to Pefloxacin (50%), Sparfloxacin, Chloramphenicol (75%), and Ciprofloxacin (75%), but resistant to Gentamicin (100%), Streptomycin (100%), Sulfamethoxazole (75%), Augmentin (75%). Escherichia coli were highly sensitive to Gentamicin (72.7%), Ciprofloxacin (100%), Ofloxacin (90.9%), Sparfloxacin (72.7%), but highly resistant to Sulphamethoxazole (72.2%), Whereas, Pseudomonas aeruginosa were highly sensitive Ciprofloxacin (100%), Chloramphenicol (66.6%), Ofloxacin (66.6%), but highly resistant against Streptomycin (100%), Augmentin 83.35%), Perfloxacin (83.3%). Data from this study suggested that raw milk contaminated with drug-resistant bacteria may cause public health hazards.