Aminogram in Egyptian Patients Experiencing Myocardial Infarction

Abstract

Background: Myocardial infarction is one of the leading causes of death worldwide. Altered amino acids metabolism is linked to cardiovascular disorders. A cross-sectional case control study was set to profile amino acids explore their potential as diagnostic and prognostic tool. Methods: Total of 60 subjects was recruited, comprising 40 myocardial infarction patients and 20 controls. High pressure liquid chromatography was applied to quantify the plasma amino acids. Results: Fourteen amino acids exhibited discriminative values between cases and controls. Leucine, isoleucine, methionine, glycine, threonine, serine, ornithine, arginine, histidine, and tyrosine exhibited low levels compared to healthy controls (p ≤0.001).  Meanwhile, cases displayed higher abundance of cysteine, taurine (p ≤ 0.05), total aromatic amino acids (p ≤ 0.01), and asparagine (p ≤ 0.001). Only leucine, isoleucine, and asparagine were correlated to GRACE risk score. Methionine and glycine scored highly (AUC > 0.95), sensitivity (>97%), and specificity (>95%) followed by histidine (AUC > 0.92), sensitivity (>82%), and specificity (>85%). Conclusion: Our study designated a panel of 14 plasma circulating amino acids. Methionine, glycine and histidine were found to be potential markers to differentiate cases from healthy Individuals. Plasma amino acids profiling could help in the diagnosis but is less powerful as a prognostic tool of Myocardial infarction.