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Research Article Open access CC BY 4.0

Ulcero-Protective Potential of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf Binary Combinations in Indomethacin Induced Gastric Ulcer

Emeka S. Asiwe, Chidi U. Igwe, Kizito M. E. Iheanacho, Viola A. Onwuliri, Chinwe S. Alisi, Nmadike G. Ezeji-Chigbu, Cosmas O. Ujowundu

Asian Journal of Biochemistry, Genetics and Molecular Biology · pp. 139–153 · Published 22 Dec 2022

10.9734/ajbgmb/2022/v12i4279

Abstract

Aims: This study was aimed at investigating the protective potential of ethyl acetate fractions of Persea americana (PA) seed and Bryophyllum pinnatum (BP) leaf binary combinations against indomethacin-induced gastric ulcers. Methodology: Fifty (50) male Wistar rats were used in this study; they were assigned into 10 groups of five animals each and respective groups received a standard rat diet and drinking water ad libitum. The groups were group 1 (normal control group (NC)), group 2 (ulcer control (UC)), group 3 (Omeprazole, 20mgkg-1 (OMEP)), group 4 (PA) and group 5 (BP). Groups receiving binary combinations were group 6 (PA + BP, 1:1), group 7 (PA + BP, 1:2), group 8 (PA + BP, 1:3), group 9 (PA + BP, 2:1), group 10 (PA + BP, 3:1) each group was pre-treated with 400 mgKg-1 body weight/day of respective fraction or binary mixture by intubation for 21 days. On the 22nd day after overnight fasting, a gastric ulcer was induced with indomethacin (30mg/kg body weight) by intubation in a single dose. Ulcer markers and histopathology of stomach tissues were measured using standard methods. Results: The present study revealed that the ethyl acetate fraction of the plants PA seeds and BP leaf showed potent gastric tissue protective effects. Analysis of gastric changes indicated a significant (P<0.05) reduction in ulcer index, gastric acid output, gastric mucus content and pepsin activity  of ulcerated rats pretreated with PA and BP fractions. The effect of the binary combinations was significantly (p<0.05) higher than single plant fractions. The protective effect of the fractions was in the order OMEP > PA+BP (3:1) > PA+BP (2:1) > PA+BP (1:1), > PA+BP (1:2) > PA+BP (1:3) > PA > BP. The observed ulcero-protective effect of the binary combinations of P. americana seed and B. pinnatum leaf may be attributed to the synergy of the phytochemicals contained in the fractions. Conclusion: These findings suggest that P. americana and B. pinnatum ethyl acetate fraction combination favorably protect against indomethacin-induced gastric mucosal oxidative damage, reducing ulcer index, gastric acid output, gastric mucus content and pepsin activity. The ethyl acetate fractions exhibited a dose-dependent inhibition of ulcer formation in indomethacin gastric ulcer model.

P. Americana B. pinnatum indomethacin gastric ulcer ulcer index

References (29)

  1. 1 PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT [DOI]
  2. 2 Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids [DOI]
  3. 3 Prostaglandins and Inflammation [DOI]
  4. 4 Gastroduodenal mucus bicarbonate barrier: protection against acid and pepsin [DOI]
  5. 5 Perforated peptic ulcer - an update [DOI]
  6. 6 Anti-oxidant, anti-inflammatory and anti-cancer activities of avocado (Persea americana) fruit and seed extract [DOI]
  7. 7 The role of nitric oxide in peptic ulcer [DOI]
  8. 8 EFFECTS OF PROPRANOLOL ON GASTRIC SECRETION IN ALBINO RATS [DOI]
  9. 9 Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy [DOI]
  10. 10 Avocado seeds (Persea americana Mill.) prevents indomethacin-induced gastric ulcer in mice [DOI]
  11. 11 Uncomplicated peptic ulcer in the UK: trends from 1997 to 2005 [DOI]
  12. 12 Structural characterization of the papaya cysteine proteinases at low pH [DOI]
  13. 13 Anti-Inflammatory and Chemopreventive Effects of Bryophyllum pinnatum (Lamarck) Leaf Extract in Experimental Colitis Models in Rodents [DOI]
  14. 14 Gastric Ulcer Healing Property of Bryophyllum pinnatum Leaf Extract in Chronic Model In Vivo and Gastroprotective Activity of Its Major Flavonoid [DOI]
  15. 15 Helicobacter pylori and perforated peptic ulcer. Prevalence of the infection and role of non-steroidal anti-inflammatory drugs [DOI]
  16. 16 Anti-ulcerogenic and proton pump (H+, K+ ATPase) inhibitory activity of Kolaviron from Garcinia kola Heckel in rodents.
  17. 17 Hypotensive and Antihypertensive Properties and Safety for Use of Annona muricata and Persea americana and Their Combination Products [DOI]
  18. 18 Potential Role for Matrix Metallo- proteinase-3 in Gastric Ulcer Healing [DOI]
  19. 19 Factors associated with gastro-duodenal disease in patients undergoing upper GI endoscopy at the Korle-Bu Teaching Hospital, Accra, Ghana [DOI]
  20. 20 Bioactive Composition and Acute Oral Toxicity Studies on Persea Americana Seed Ethyl Acetate Fraction [DOI]
  21. 21 ANTIOXIDANT AND ANTIMICROBIAL ACTIVITIES OF BRYOPHYLLUM CALYCINUM SALISB LEAF
  22. 22 Ulcer Healing Effect Of Anti-Ulcer Agents: A Comparative Study [DOI]
  23. 23 Characterization of Chemical Composition of Bryophyllum pinnatum Leaf Ethyl Acetate Fraction [DOI]
  24. 24 Effects of free radical scavengers on indomethacin-induced aggravation of gastric ulcer in rats [DOI]
  25. 25 OXIDATIVE STRESS IMPLICATIONS IN EXPERIMENTAL GASTRIC ULCER INDUCED BY INDOMETHACIN [DOI]
  26. 26 Antioxidative and Free Radical Scavenging Properties of Ethyl Acetate Fractions of Persea americana Seed and Bryophyllum pinnatum Leaf [DOI]
  27. 27 Ulcer-Protective Property of Bryophyllum pinnatum Leaf Extract and their Phytosomal Formulations [DOI]
  28. 28 Antiulcer effect of Persea americana seed against alcohol-induced peptic ulcer in guinea pig
  29. 29 Papaya (Carica papaya L.) Biology and Biotechnology

Cited by 2

Persea americana seed and Bryophyllum pinnatum leaf ethyl acetate binary combinations protects against non-steroidal anti-inflammatory drug-induced oxidative stress

E. S. Asiwe, U. E. Enenebeaku, P. N. Alisi · Journal of Pharmacognosy and Phytochemistry · 2024

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