The Simultaneous Determination of Some Water-Soluble Vitamins in Gum of Acacia nilotica by High Performance Liquid Chromatography
Sarra Bouazizi, Bassem Jamoussi, Dalila Bousta
Advances in Research · pp. 312–318 · Published 24 Oct 2014
10.9734/AIR/2015/10976Abstract
A rapid, simple and precise method by HPLC (high performance liquid chromatography) has been developed for simultaneous determination of water-soluble vitamins as thiamine(B1), nicotinamide(B3), panthotenic(B5), pyridoxine(B6) and biotin(B8) in gum of Acacia nilotica using enzymatic hydrolysis. The method uses a C18 column (4.6×150 mm, 5µm). Mobile phase such as methanol 0.1M, sodium dihydrogen phosphate (pH = 2.5), (10:90 v/v) is found most suitable for rapid separation and identification of this water –soluble vitamins. Good linearity was observed between the concentration of analytes and peak area (r = 0.9999). Each vitamin was quantitatively determined at its maximum wavelength. Recovery percentages ranged from 97% to 99%.
References (20)
- 1 Determination of eight water- and fat-soluble vitamins in multi-vitamin pharmaceutical formulations by high-performance liquid chromatography [DOI]
- 2 Plasma amino acids determined by liquid chromatography within 17 minutes [DOI]
- 3 Determination of water-soluble vitamins in infant milk by high-performance liquid chromatography [DOI]
- 4 Production of lactic acid by Lactobacillus rhamnosus with vitamin-supplemented soybean hydrolysate [DOI]
- 5 Chromatographic determination of vitamins in foods [DOI]
- 6 Development of a validated HPLC method for the determination of B‐complex vitamins in pharmaceuticals and biological fluids after solid phase extraction [DOI]
- 7 Development of a validated liquid chromatography method for the simultaneous determination of eight fat-soluble vitamins in biological fluids after solid-phase extraction [DOI]
- 8 An analysis of avidin, biotin and their interaction at attomole levels by voltammetric and chromatographic techniques [DOI]
- 9 Simultaneous determination of water-soluble vitamins excreted in human urine after eating an overdose of vitamin pills by a HPLC method coupled with a solid phase extraction [DOI]
- 10 Precise analysis of primary amino acids in urine by an automated high-performance liquid chromatography method: comparison with ion-exchange chromatography [DOI]
- 11 Flow-injection solvent extraction without phase separation [DOI]
- 12 Quantitative determination of vitamin B6 in dietary foods for special medical purposes by microbiological assay method [DOI]
- 13 Voltammetric Behavior and Determination of Tocopherol in Vegetable Oils at a Polypyrrole Modified Electrode [DOI]
- 14 Quantitative gas-liquid chromatography of sulfur containing amino acids [DOI]
- 15 Simultaneous determination of total and extracellular concentrations of the amino acid neurotransmitters in cat visual cortex by microbore liquid chromatography and electrochemical detection [DOI]
- 16 HPLC Analysis of Taurine in Human Plasma Sample Using the Dabs-Ci Reagent with Sensitivity at Picomole Level [DOI]
- 17 A high-speed liquid chromatograph with a flow-spectrofluorimetric detector and the ultramicro-determination of aromatic compounds [DOI]
- 18 Electron-capture gas chromatography of taurine as its N-pentafluorobenzoyl di-n-butylamide derivative [DOI]
- 19 Quantification of the characteristics of antagonists exhibiting both competitive antagonism and functional interaction [DOI]
- 20 Determination of taurine metabolism by measurement of 15N-enriched taurine in cat urine by gas chromatography—mass spectrometry [DOI]
Cited by 4
Dalel Melki, Lassaad Hedhili, L. Hamrouni · 2018
Showing 1 of 4 known citations — external sources report more than can currently be individually listed.
Related research
- Water, Sanitation and Hygiene Practices among Households in Teshie, Accra, Ghana — shares topic coverage
- Physicochemical and Microbiological Parameters of Water from Rivers in Keffi, Central Nigeria — shares topic coverage
- A Comparative Study of the Bioremediation Potentials of Inorganic Nutrient Sources — shares topic coverage
- Effect of Convergent Rays on Coliform and Total Culturable Heterotrophic Bacteria in Water — shares topic coverage
- Molecular Detection of Giardia lamblia and Cryptosporidium parvum in Different Water Sources of District Bannu, Khyber Pakhtunkhwa, Province of Pakistan — shares topic coverage
Article metrics
Real usage data collected on this platform.
29
Page views
0
PDF downloads
4
Outbound clicks
4
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.