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Research Article Open access CC BY 3.0

Molecular Detection of Giardia lamblia and Cryptosporidium parvum in Different Water Sources of District Bannu, Khyber Pakhtunkhwa, Province of Pakistan

Muhammad Shoaib Alam, Sana Ullah Khan, Sultan Ayaz, Noor Ul Akbar, Muhammad Asim Khan, Iftikhar Ahmad, Muhammad Idrees, Muhammad Waqar

Microbiology Research Journal International · pp. 80–88 · Published 9 Oct 2013

10.9734/BMRJ/2014/3947

Abstract

Background: Cryptosporidium parvum and Giardia lamblia are intestinal parasites that predominantly causes "waterborne" infections that are transmitted through consumption of contaminated water. Both parasites typically cause an acute short-term infections with self-limiting diarrhea as the main symptom in people with intact immune systems. However, in immunocompromised individuals, the symptoms are particularly severe and might be fatal. Methods: The study was carried out in District Bannu Khyber Pukhtunkhwa, Pakistan for the detection of G. lamblia and C. parvum parasites in drinking water in different villages/localities (Kakki, Jamon Road, Kotka Juma Khan, Sokari, Mandan and Bannu City). Water samples n=75 were collected from different water sources between 1st August 2011 to 30th January 2012. These samples included tap, pond, borewell and hand pump water that were filtrated and residue was subjected to amplify by PCR. Results: Overall prevalence of parasites was 36% (25/75), containing tap 17.64% (9/51) and pond water 75% (6/8), bore well water 41.66% (5/12) and hand pump water 50% (2/4). Similarly over all prevalence rate of tap water for C. parvum was 7.84% (4/51) while for G. lamblia was 9.80% (5/51) positive. The present study revealed that the people of the area should use the cleaned and filtered water. Conclusion: Contamination of water with G. lamblia and C. parvum was found in water sources especially the drinking ones, of District Bannu which need proper water treatment to decontaminate and large scale studies are needed.

G. lambli C. parvum diarrhea water PCR

References (20)

  1. 1 Giardia and Cryptosporidium join the ‘Neglected Diseases Initiative’ [DOI]
  2. 2 Cryptosporidium: a water-borne zoonotic parasite [DOI]
  3. 3 The zoonotic significance and molecular epidemiology of Giardia and giardiasis [DOI]
  4. 4 Zoonotic protozoa: from land to sea [DOI]
  5. 5 Variation in Giardia: Implications for Taxonomy and Epidemiology [DOI]
  6. 6 Giardia and Cryptosporidium in mammalian wildlife – current status and future needs [DOI]
  7. 7 The potential for marine bivalve shellfish to act as transmission vehicles for outbreaks of protozoan infections in humans: A review [DOI]
  8. 8 Occurrence of Giardia and Cryptosporidium in water supplies of Russia and Bulgaria [DOI]
  9. 9 Comparison of primers and optimization of PCR conditions for detection of Cryptosporidium parvum and Giardia lamblia in water [DOI]
  10. 10 Cryptosporidiosis: A Global Challenge [DOI]
  11. 11 MixedCryptosporidiumInfections and HIV [DOI]
  12. 12 Prevalence of Cryptosporidium spp. and Giardia spp. in Five Marine Mammal Species [DOI]
  13. 13 Genotype of Giardia intestinalis isolates from children and dogs and its relationship to host origin [DOI]
  14. 14 Socio-Economic Factors Associated with Intestinal Parasites Among Children Living in Gombak, Malaysia [DOI]
  15. 15 HIV1 and the gut in the era of highly active antiretroviral therapy [DOI]
  16. 16 Molecular identification of a Cryptosporidium saurophilum from corn snake (Elaphe guttata guttata) [DOI]
  17. 17 Prevalence of zoonotic parasites in drinking water of three districts of Khyber Pakhtunkhwa Province, Pakistan
  18. 18 Apicomplexan parasites: environmental contamination and transmission.
  19. 19 STATUS OF GIARDIA INTESTINALIS INFECTION AMONG THE CHILDREN ATTENDING KANTI CHILDREN HOSPITAL, NEPAL
  20. 20 Prevalence and Molecular Detection of Giardia and Cryptosporidium spp in Communities Consuming Different Drinking Water Sources in Kohat, Khyber Pakhtunkhwa Pakistan

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