Skip to content
Research Article Open access CC BY 4.0

Phytochemical Screening for Medicinal Plants: Guide for Extraction Methods

D. S. H. S. Peiris, D. T. K. Fernando, S. P. N. N. Senadeera, C. B. Ranaweera

Asian Plant Research Journal · pp. 13–34 · Published 16 Jun 2023

10.9734/aprj/2023/v11i4216

Abstract

Sri Lankans have relied on traditional medicine to meet their primary health needs since the beginning of time. This island is blessed with an enormous number of medicinal plants, which play a very important role in traditional medicine. However, the lengthy documented history of traditional medicine is still composed of medicinal plants, which are not scientifically proven to have the mentioned abilities or activities. To obtain scientifically sound information from this documented history of traditional medicine, extraction of the biologically active compounds from these medicinal plants is very important. Also, to maintain the accuracy of results obtained from in vitro and in vivo assays, it is important to consider the pre-extraction procedures as well as the evaporation and storage conditions of the extract. There are several extraction methods accessible in Sri Lanka. This research aims to review the pre-extraction preparation, extraction methods, evaporation techniques, and storage conditions of the plant extract. This review highlights that the reliability of phytochemical screening results is affected by the identification and authentication of the plant, pre-extraction procedures, menstruum utilized, method of extraction, and storage conditions.

Sri Lanka extraction methods phytochemical screening secondary metabolites bio-active compounds maceration medicinal plants identification and authentication

References (50)

  1. 1 Biodiversity hotspots for conservation priorities [DOI]
  2. 2 Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts [DOI]
  3. 3 Phytochemical screening and Extraction: A Review
  4. 4 Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes [DOI]
  5. 5 A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation [DOI]
  6. 6 Plant Secondary Metabolite Biosynthesis and Transcriptional Regulation in Response to Biotic and Abiotic Stress Conditions [DOI]
  7. 7 Concept of standardization, extraction and pre phytochemical screening strategies for herbal drug
  8. 8 Comparison of different extraction methods for the extraction of major bioactive flavonoid compounds from spearmint (Mentha spicata L.) leaves [DOI]
  9. 9 Effect of solvents in extracting polyphenols and antioxidants of selected raw vegetables [DOI]
  10. 10 Plant growth regulator mediated consequences of secondary metabolites in medicinal plants [DOI]
  11. 11 Developing as Culturally Responsive Mathematics Teacher Educators: Reviewing and Framing Perspectives in the Research [DOI]
  12. 12 Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents [DOI]
  13. 13 Phytochemicals: Extraction methods, identification and detection of bioactive compounds from plant extracts
  14. 14 Modern Methods of Pre-Treatment of Plant Material for the Extraction of Bioactive Compounds [DOI]
  15. 15 Antimicrobial Activity of Plant Secondary Metabolites: A Review [DOI]
  16. 16 Anti-inflammatory activity of Trichodesma indicum root extract in experimental animals [DOI]
  17. 17 Chemical Changes of Bioactive Phytochemicals during Thermal Processing [DOI]
  18. 18 Effect of solvent evaporation method on phenolic compounds and the antioxidant activity of Moringa oleifera cultivated in Southern Tunisia [DOI]
  19. 19 Isolation, fractionation and identification of chemical constituents from the leaves crude extracts of Mentha piperita L grown in Sultanate of Oman [DOI]
  20. 20 Overview of Alternative Ethanol Removal Techniques for Enhancing Bioethanol Recovery from Fermentation Broth [DOI]
  21. 21 Supercritical Fluid Extraction : A Review
  22. 22 Cosmos Caudatus as a Potential Source of Polyphenolic Compounds: Optimisation of Oven Drying Conditions and Characterisation of Its Functional Properties [DOI]
  23. 23 Comparative studies on antimicrobial properties of extracts of fresh and driedleaves of Carica papaya (L) on clinical bacterial and fungal isolates
  24. 24 Aqueous based reflux method for green synthesis of nanostructures: Application in CZTS synthesis [DOI]
  25. 25 Anti-inflammatory activity of roots ofAchyranthes aspera [DOI]
  26. 26 Storage Effect on Phenols and on the Antioxidant Activity of Extracts from Anemopsis californica and Inhibition of Elastase Enzyme [DOI]
  27. 27 Phenolic Contents and Antioxidant Activity of Extracts of Selected Fresh and Dried Herbal Materials [DOI]
  28. 28 Effect of power ultrasound on the extraction of black caraway (Carum carvi L.) and evaluation of their qualitative properties using response surface methodology [DOI]
  29. 29 Extraction, Storage Duration, and Storage Temperature Affect the Activity of Ascorbate Peroxidase, Glutathione Reductase, and Superoxide Dismutase in Rice Tissue [DOI]
  30. 30 Mirabilis jalapa Linn.: A Folklore Ayurvedic Medicinal Plant in Sri Lanka [DOI]
  31. 31 Supercritical CO2 fractionation of crude palm oil [DOI]
  32. 32 Antioxidant properties and cytotoxicity evaluation of methanolic extract of dried and fresh rhizomes of Tulbaghia violacea [DOI]
  33. 33 Evaluating the effect of storage on the biological activity and chemical composition of three South African medicinal plants [DOI]
  34. 34 Anti-inflammatory activity of roots of Ecbolium viride (Forsk) Merrill [DOI]
  35. 35 Preliminary study: kinetics of oil extraction from sandalwood by microwave-assisted hydrodistillation [DOI]
  36. 36 Ionic liquids as green solvents in herbal extraction
  37. 37 Extraction of Caffeine [DOI]
  38. 38 The Role of Secondary Metabolites Obtained from Medicinal and Aromatic Plants in Our Lives [DOI]
  39. 39 Clitoria ternatea - Shifting Paradigms: From Laboratory to Industry [DOI]
  40. 40 Lack of in vitro antihyaluronidase activity of methanolic leaf extract of Indigofera tinctoria L and methanolic stem bark extract of Stereospermum suaveolens DC [DOI]
  41. 41 Rich Diversity & Potential Medicinal Value of Endemic Sri Lankan Plant: Jeffreycia zeylanica [DOI]
  42. 42 Utilization of various parts of the plant for the extraction of phytochemicals and high-throughput screening techniques [DOI]
  43. 43 Effect of Solvent Evaporation Methods on Biological Activity of Crude Extract of Feronia elephantum Correa Leaves [DOI]
  44. 44 Medicinal Plants of Sri Lanka
  45. 45 Mechanisms of Introduction Into the Environment [DOI]
  46. 46 Indian Medicinal Plants [DOI]
  47. 47 Journal of Pharmacognosy and Phytochemistry [DOI]
  48. 48 Internationale Pharmaceutica Sciencia [DOI]
  49. 49 International Journal of Advanced Research and Development [DOI]
  50. 50 Influence of Sample Preparation/Extraction Method on the Phytochemical Profile and Antimicrobial Activities of 12 Commonly Consumed Medicinal Plants in Romania [DOI]

Cited by 36

A Comprehensive Review of Salacia reticulata: Botanical, Ethnomedicinal, Phytochemical, and Pharmacological Insights

D. S. H. S. Peiris, D. T. K. Fernando, S. P. N. N. Senadeera, C. B. Ranaweera · Asian Plant Research Journal · 2023

In vitro Anti-Inflammatory Activity of Leaves of Jeffreycia zeylanica Using the Egg Albumin Denaturation Method and Human Red Blood Cell Stabilization Method

Thummini Samaraweera, Thumuli Samaraweera, Nimesha N. Senadeera, Chathuranga B. Ranaweera · Asian Plant Research Journal · 2023

Therapeutic Potential of Carica papaya Linn.: A Comprehensive Review of Its Pharmacological Properties and Health Benefits, with Emphasis on Dengue Fever Healing

D. T. K. Fernando, D. S. H. S. Peiris, S. P. N. N. Senadeera, C. B. Ranaweera · Asian Plant Research Journal · 2024

Phytochemical Testing Methodologies and Principles for Preliminary Screening/ Qualitative Testing

Luvedika Maheshwaran, Ladhurshika Nadarajah, S. P. N. N. Senadeera, C. B. Ranaweera, A. K. Chandana, R. N. Pathirana · Asian Plant Research Journal · 2024

Chemical Compound Profile of Infusion from Crescentia cujete Fruit Peel and Toxicity Test against Artemia sallina Leach

Rahma Diyan Martha, Maya Sri Ashira, Choirul Huda · Biology, Medicine, & Natural Product Chemistry · 2026

Evaluation of Anti-Psoriatic Activity of Aloe sinkatana Extract on Imiquimod Induced Psoriatic-Like Dermatitis in Mice

A. Dawoud, sali Dawoud, Mohammed Abdalbagi · Plant Biotechnology Persa · 2026

Showing 23 of 36 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

130

Page views

0

PDF downloads

4

Outbound clicks

36

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.