25 (OH) Vitamin D level and Calcium/Phosphorus Metabolism Disorders in Patients Living with HIV in Abidjan

Abstract

Aims: The present study was to evaluate the serum calcium level, phosphorus and 25 (OH) vitamin D subjects during HIV infection; precisely in patients living with HIV (PLHIV). Study Design: Prospective and experimental study, involving a cohort of blood samples from HIV-negative and positive subjects. Place and Duration: The study was carried out in the clinical and fundamental biochemistry Department of the Institut Pasteur of Côte d’Ivoire from June 2014 to December 2014. Methodology: A cohort of 220 samples from adult blood samples, comprising of 110 HIV negative controls and 110 samples from PLHIV subjects, were analyzed. After confirmation of HIV status (through an HIV rapid test) of subjects, CD4 lymphocytes were measured through flow cytometry analysis using an automated FacsCaliburTM. Analysis of calcium and phosphorus electrolytes was performed on the Cobas Integra 400 Plus and 25 (OH) vitamin D on the automated mini VIDAS, from the sera obtained from different blood samples from HIV positive and negative subjects. Results: The identified disorders in the PLHIV were hypocalcemia (P = 0.0009) and hyperphosphoremia (p < 0.0001). 57% of PLHIV against 67% of the control subjects showed normal levels of 25 (OH) vitamin D (P > 0.05). Conclusion: We noticed a good level of 25 (OH) Vitamin D reserve in PLHIV among more than 50% of PLHIV. Calcium and Phosphorous metabolism disorders observed in people living with HIV in Côte d'Ivoire are due to a deficiency of the active form 1,25 (OH)2 vitamin D.