Skip to content
Research Article Open access CC BY 4.0

Larvicidal Efficacy and GC-MS Analysis of Hyptis suaveolens Leaf Extracts against Anopheles Species

Y. D. Dakum, C. N. Amajoh, A. Ombugadu, G. Istifanus, F. Agwom, S. T. Joseph, I. R. Jwanse, P. M. Lapang, S. W. Kopdorah, D. D. Pam

International Journal of Biochemistry Research & Review · pp. 8–19 · Published 2 Mar 2021

10.9734/ijbcrr/2021/v30i130241

Abstract

Mosquitoes are the most important group of insects in terms of public health importance, which transmit serious human diseases. The continuous application of synthetic insecticides to control these mosquitoes causes development of resistance in vector species, and an adverse effect on environmental quality and non-target organisms including human health. Therefore, the use of active toxic agents from plant extracts as alternative mosquito control strategy cannot be over emphasized, as these are non-toxic, easily available at affordable prices, biodegradable and show target - specific activities against different species of vector mosquitoes. The aim of the present study was to evaluate the larvicidal efficacy of the aqueous and methanol leaf extracts of H. suaveolens against the fourth instars larvae of Anopheles species. Standard WHO protocols was adopted for the larvicidal bioassay. Larvicidal activity was evaluated at concentrations of 200, 400, 600, 800 and 1000 mg/L. The larval mortality was observed after 24 and 48hours bioassay. The results are mean of three replicates and the mortalities recorded were subjected to chi-square test and probit analysis to determine median lethal concentrations (LC50). TheGC-MS analysis ofthe methanolic leaf extract of the plantrevealed the presence of twelve compounds on the chromatogram. Among the major compounds areOleic Acid (33.33%), Octadecanoic acid (13.52%), 1,3-Cyclohexadiene-1-methanol,alpha.,2,6,6-(10.42%), 1,3-Cyclopentanediol, trans (9.60%), n-Hexadecanoic acid (9.01%) and 4-Hepten-3-one, 4-methyl (7.0%).The phytochemical screening of the aqueous and methanol leaf extracts of the plant revealed the presence of Tannins, Terpenoids, Flavonoids, Steroids, Carbohydrates, Cardiac glycoside and resins. The LC50 values estimated were 316.22mg/Lfor methanol extract and 323.59 mg/L for aqueous extract. The differences between the two extracts were however not significantly different at P>0.05. The findings also revealed that mortality was concentration dependent and both extracts showed promising larvicidal activity against Anopheles species larvae. Therefore, the crude leaf extract of the plant is recommended to be used as alternative to synthetic larvicides and further research is needed to test the activity of the plant on non-target organisms.

Mosquito anopheles larvae Hyptis suaveolens extracts

References (22)

  1. 1 Medicinal plants and traditional medicine in Africa
  2. 2 MALARIA TRANSMISSION IN URBAN SUB-SAHARAN AFRICA [DOI]
  3. 3 Advantages of larval control for African malaria vectors: Low mobility and behavioural responsiveness of immature mosquito stages allow high effective coverage [DOI]
  4. 4 Mosquito larvicidal activity of oleic and linoleic acids isolated from Citrullus colocynthis (Linn.) Schrad [DOI]
  5. 5 Mosquito larvicidal activities of Solanum villosum berry extract against the dengue vector Stegomyia aegypti [DOI]
  6. 6 Larvicidal activities of three plants against filarial vector Culex quinquefasciatus Say (Diptera: Culicidae) [DOI]
  7. 7 Dinotefuran: A Potential Neonicotinoid Insecticide Against Resistant Mosquitoes [DOI]
  8. 8 Repellency of live potted plants against Anopheles gambiae from human baits in semi-field experimental huts. [DOI]
  9. 9 RATIONALIZING HISTORICAL SUCCESSES OF MALARIA CONTROL IN AFRICA IN TERMS OF MOSQUITO RESOURCE AVAILABILTY MANAGEMENT [DOI]
  10. 10 Repellent activity of herbal essential oils against Aedes aegypti (Linn.) and Culex quinquefasciatus (Say.) [DOI]
  11. 11 Evaluation of Larvicidal Effect of Lantana Camara Linn Against Mosquito Species Aedes aegypti and Culex quinquefasciatus
  12. 12 Insecticidal activity of extracts derived from different parts of the mangrove tree rhizophora mucronata (rhizophoraceae) lam. Against three arthropods [DOI]
  13. 13 Evaluation of larvicidal activity of the leaf extract of a weed plant, Ageratina adenophora, against two important species of mosquitoes, Aedes aegypti and Culex quinquefasciatus [DOI]
  14. 14 Phyto-Synthesized Silver Nanoparticles: A Potent Mosquito Biolarvicidal Agent [DOI]
  15. 15 Evaluation of mosquito repellent activity of isolated oleic acid, eicosyl ester from Thalictrum javanicum [DOI]
  16. 16 Larvicidal efficacy of medicinal plant extracts against the vector mosquito Aedes albopictus
  17. 17 Mosquito larvicidal activity of Hyptis suaveolens (L.) Poit (Lamiaceae) aerial extracts against the filarial vector Culex quinquefasciatus Say (Diptera: Culicidae)
  18. 18 Adulticidal Effects of Essential Oils Extracts from Capsicum annuum (Solanaceae) Piper nigrum (Piperaceae) and Zingiber officinale (Zingiberaceae) on Anopheles gambiae (Diptera-Culicidea), Vector of Malaria [DOI]
  19. 19 Changes in Haematological Parameters of Clarias gariepinus Exposed to Century Plant (Agave americana) Leaf Dust
  20. 20 Larvicidal Activity of a New Glycoside, Phenyl Ethyl β-D Glucopyranoside from the Stem of the Plant Sida rhombifolia [DOI]
  21. 21 Malaria control in the context of integrated management of childhood illness in Tanzania: the challenges ahead [DOI]
  22. 22 Laboratory Evaluation of Pesticidal Activities of Hyptis suaveolens in Pest Management [DOI]

Cited by 6

GC–MS analysis and assessment of insecticidal properties of Anacardium occidentale leaf extracts

Eze Elijah Ajaegbu, Gloria Tochukwu Onah, Abdulrasheed Momoh Bello · International Journal of Tropical Insect Science · 2025

Bioactive molecules derived from Scoparia dulcis medicinal flora: Act as a powerful bio-weapon against agronomic pests and eco-friendlier tool on non-target species

Dinakaran Vasumathi, Swaminathan Senguttuvan, Jeganathan Pandiyan · South African Journal of Botany · 2023

Showing 2 of 6 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

29

Page views

0

PDF downloads

2

Outbound clicks

6

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.