Skip to content
Research Article Open access CC BY 3.0

Biological and Reproduction Activities of Mosquito Larvae of Culiseta morsitans (Theobald) after Treatment by Ecdysone Agonist Methoxyfenozide

Jaouida Haouari-Abderrahim, Nassima Rehimi

Annual Research & Review in Biology · pp. 4152–4165 · Published 30 Jul 2014

10.9734/ARRB/2014/11366

Abstract

Aims: Insect growth regulators (IGRs) offer alternatives to conventional chemical larvicides that pose problem of resistance and environmental safety. However, only a limited number of IGRs have been approved for use in mosquito control. The present investigation was to evaluate the insecticidal activity of a non-steroidal ecdysteroid agonist, methoxyfenozide (RH-2485), against last-instar larvae of Culiseta morsitans (Culicidae: Diptera) under the laboratory conditions, and its effect on fecundity and fertility of females. Methodology: Based on preliminary bioassays, five concentrations ranging from 0.012 to 0.072mg/L were tested. The larvicidal test was made according to the World Health Organization (WHO) protocol standard by exposing newly ecdysed fourth- instar larvae for 24h. CL50 value of methoxyfenozide value was determined by probit analysis. Results: The insecticidal activity of methoxyfenozide result in a dose- dependent reduction in adult emergence. In addition, larval mortality was relatively important compared with the mortality recorded for the other following developmental stages. The larval mortality varies between 16.84±3.89 % and 66.24±3.50% for 0.012 mg/L and 0.072 mg/L, respectively, and LC50 value was 0.059mg/L. The ecdysteroidal activity of this compound caused premature induction of larval moulting and incomplete pupation. Also, the different types of morphogenetic aberrations in the larva, pupa and adult after exposure to methoxyfenozide are illustrated (larval-pupal and pupal-adult). Moreover, the tested compound greatly affected the reproductive parameters of surviving females derived from larval treatment. Methoxyfenozide caused a significant reduction of fecundity and fertility (P<0,001). Also, no egg laying has been recorded with the higher concentration 0.072 mg/L, which produced 100% of sterility. Conclusion: Methoxyfenozide is an efficient insect growth regulator for controlling mosquito populations. This compound may prove to be an effective larvicide for treatment of mosquito, to decrease the frequency of pathogen transmission to humans and animals.

Toxicity mosquito Culiseta morsitans ecdysteroid agonist methoxyfenozide abnormalities fecundity

References (45)

  1. 1 A Method of Computing the Effectiveness of an Insecticide [DOI]
  2. 2 Probit Analysis (3rd ed). [DOI]
  3. 3 NEW INSECTICIDES WITH ECDYSTEROIDAL AND JUVENILE HORMONE ACTIVITY [DOI]
  4. 4 Action of a novel nonsteroidal ecdysteroid mimic, tebufenozide (RH‐5992), on insects of different orders [DOI]
  5. 5 Toxicity and kinetics of methoxyfenozide in greenhouse‐selected Spodoptera exigua (Lepidoptera: Noctuidae) [DOI]
  6. 6 Lethal and Sublethal Effects of Methoxyfenozide and Spinosad on Spodoptera littoralis (Lepidoptera: Noctuidae) [DOI]
  7. 7 Pro-active Management of Beet Armyworm (Lepidoptera: Noctuidae) Resistance to Tebufenozide and Methoxyfenozide: Baseline Monitoring, Risk Assessment, and Isolation of Resistance [DOI]
  8. 8 The Significance of Pharmacokinetics and Metabolism to the Biological Activity of RH-5992 (Tebufenozide) in Spodoptera exempta, Spodoptera exigua, and Leptinotarsa decemlineata [DOI]
  9. 9 Chemosterilization of Larvae and Adults of the Egyptian Cotton Leafworm, Prodenia litura, by Apholate, Metepa, and Tepa [DOI]
  10. 10 Toxicity and Pharmacokinetics of Insect Growth Regulators and Other Novel Insecticides on Pupae of Hyposoter didymator (Hymenoptera: Ichneumonidae), a Parasitoid of Early Larval Instars of Lepidopteran Pests [DOI]
  11. 11 Bisacylhydrazine Insecticides for Selective Pest Control [DOI]
  12. 12 The ecological impact of four IGR insecticides in adults of Hyposoter didymator (Hym., Ichneumonidae): pharmacokinetics approach [DOI]
  13. 13 Larvicidal Activity of a Neem Tree Extract (Azadirachtin) Against Mosquito Larvae in the Republic of Algeria
  14. 14 Comparative larvicidal toxicities of three ecdysone agonists on the mosquitoes Aedes aegypti, Culex quinquefasciatus, and Anopheles gambiae [DOI]
  15. 15 Comparative toxicity and ecdysone receptor affinity of non-steroidal ecdysone agonists and 20-hydroxyecdysone in Chironomus tentans [DOI]
  16. 16 Toxicity and Pharmacokinetics of Spinosad and Methoxyfenozide toSpodoptera littoralis(Lepidoptera: Noctuidae) [DOI]
  17. 17 Lethal and Sublethal Effects of Methoxyfenozide on the Development, Survival and Reproduction of the Fall Armyworm, Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) [DOI]
  18. 18 Sublethal Effects of Selected Insecticides on Growth and Reproduction of a Laboratory Susceptible Strain of Tufted Apple Bud Moth (Lepidoptera: Tortricidae) [DOI]
  19. 19 Azadirachtin potentiates the action of ecdysteroid agonist RH-2485 in Spodoptera littoralis [DOI]
  20. 20 Tebufenozide Targeted Against Codling Moth (Lepidoptera: Tortricidae) Adults, Eggs, and Larvae [DOI]
  21. 21 Laboratory evaluation of Alsystin, a chitin synthesis inhibitor, against Culex pipiens pipiens L. (Dip., Culicidae): effects on development and cuticle secretion [DOI]
  22. 22 Effects of ecdysone agonist halofenozide against Culex pipiens [DOI]
  23. 23 Effect of ecdysone agonist RH-0345 on reproduction of mealworm, Tenebrio molitor [DOI]
  24. 24 Effects of the insect growth regulators OMS 2017 and diflubenzuron on the reproductive potential of Aedes aegypti.
  25. 25 The biological effects of the insect growth regulators; pyriproxyfen and diflubenzuron on the mosquito Aedes aegypti.
  26. 26 Development of a novel lepidopteran insect control agent, chromafenozide [DOI]
  27. 27 Tebufenozide distorted codling moth larval growth and reproduction, and controlled field populations [DOI]
  28. 28 Laboratory Evaluation of a Biorational Insecticide, Kinoprene, against Culex pipiens Larvae: Effects on Growth and Development [DOI]
  29. 29 Sublethal Effects of Chronic Exposure to Tebufenozide on the Development, Survival, and Reproduction of the Tufted Apple Bud Moth (Lepidoptera: Tortricidae) [DOI]
  30. 30 Low field-relevant tebufenozide concentrations affect reproduction in Chironomus riparius (Diptera: Chironomidae) in a long-term toxicity test [DOI]
  31. 31 Comparative toxicity of three ecdysone agonist insecticides against the Mediterranean flour moth.
  32. 32 Statistical methods in malaria eradication.
  33. 33 Methoprene tolerance in Aedes nigromaculis in Fresno County, California.
  34. 34 Effects of Diflubenzuron on Chaoborus astictopus and Nontarget Organisms and Persistence of Diflubenzuron in Lentic Habitats1 [DOI]
  35. 35 Effect of RH-5992 on adult development in the spruce budworm, Choristoneura fumiferana [DOI]
  36. 36 Action and pharmacokinetics of a novel insect growth regulator, halofenozide, in adult beetles ofAubeonymus mariaefranciscae andLeptinotarsa decemlineata [DOI]
  37. 37 Effect of the ecdysone agonists, RH-2485 and tebufenozide, on the southwestern corn borer,Diatraea grandiosella [DOI]
  38. 38 Ecdysteroid and Juvenile Hormone Receptors: Properties and Importance in Developing Novel Insecticides [DOI]
  39. 39 Selection, Egg Viability, and Fecundity of the Sugarcane Borer (Lepidoptera: Crambidae) with Tebufenozide [DOI]
  40. 40 Tebufenozide effects on the reproductive potentials of the mediterranean flour moth, Ephestia kuehniella.
  41. 41 Action of RH 5849, a non‐steroidal ecdysteroid mimic, on Plodia interpunctella (Hübner) in vivo and in vitro [DOI]
  42. 42 Toxicity of two ecdysone agonists, halofenozide and methoxyfenozide, against the multicoloured Asian lady beetle Harmonia axyridis (Col., Coccinellidae) [DOI]
  43. 43 Bioefficacy of Azadirachtin in Controlling Culex pipiens pipiens ( Diptera : Culicidae ) [DOI]
  44. 44 The Horn Fly: Methoprene in Drinking Water of Cattle for Control12 [DOI]
  45. 45 Synergistic Effects of Verbutin with Dibenzoylhydrazine-Type Ecdysteroid Agonists on Larvae of Aedes aegypti (Diptera: Culicidae) [DOI]

Cited by 9

20-Hydroxyecdysone (20E) signaling as a promising target for the chemical control of malaria vectors

Elodie Ekoka, Surina Maharaj, L. Nardini · Parasites & Vectors · 2021

Investigation of methoxyfenozide insecticide genotoxicity with Allium and SMART methods

Fahrettin A. Sırlıbaş, Zübeyde Kumbıçak · Spanish Journal of Agricultural Research · 2025

Showing 5 of 9 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

59

Page views

0

PDF downloads

23

Outbound clicks

9

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.