Induced; Thermal Influence on Morphology of Aspergillus carbonarius During Raw Starch Digesting Amylase Production
Onyetugo C. Amadi, Bartholomew N. Okolo
Microbiology Research Journal International · pp. 355–367 · Published 3 Jun 2013
10.9734/BMRJ/2013/2815Abstract
Aim: To investigate increased thermal influence on morphology of Aspergillus carbonarius during RSDA production. Place and Duration of Study: Microbial fermentation Unit, Department of Microbiology, Faculty of Biological sciences, University of Nigeria, between July 2009 and August 2010. Methodology: In shake flask cultures thermal influence on A. carbonarius morphology and productivity investigated. Mycelial morphology was characterised by means of image analysis using as parameters, mean diameter, roughness, circularity and compactness of pellet. Thermal effect on amylase activity, total protein, biomass concentration and pH were also investigated. Results: Shifting the temperature from 27ºC to 37ºC significantly affected the morphological parameters of the pellets, but RSDA activity was not altered. The interesting thing about the morphology is the shearing off of the hairy part of the pellet at an increased temperature and subsequent agglomeration. At 27ºC the RSDA activity increased steadily with an optimum activity of 293U/ml at 96h and subsequently decreased to 75U/ml by the end of the fermentation. At 37ºC a maximum activity of 291U/ml was achieved at 72h of fermentation but this decreased to 87U/ml at the end of fermentation. Higher biomass concentration and total protein were obtained at 37ºC. The pH dropped from an initial of 5.0 to 3.0 and 2.5 for 27ºC and 37ºC temperature conditions respectively. Conclusion: Induced thermal increase resulted to changes in pellet morphology but raw starch digesting amylase activity was not altered.
References (36)
- 1 A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye Binding [DOI]
- 2 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
- 3 Brock Biology of Microorganisms
- 4 Fungal morphology and metabolite production in submerged mycelial processes [DOI]
- 5 The growth of molds in the form of pellets–a literature review [DOI]
- 6 Localization of growth and secretion of proteins in Aspergillus niger [DOI]
- 7 Mycelial morphology and metabolite production [DOI]
- 8 Morphology engineering - Osmolality and its effect on Aspergillus niger morphology and productivity [DOI]
- 9 Protein secretion in filamentous fungi — trying to understand a highly productive black box [DOI]
- 10 Morphology and physiology of an α-amylase producing strain of Aspergillus oryzae during batch cultivations [DOI]
- 11 Serine/Threonine Protein Kinases and Phosphatases in Filamentious Fungi [DOI]
- 12 Morphological development of Aspergillus niger in submerged citric acid fermentation as a function of the spore inoculum level. Application of neural network and cluster analysis for characterization of mycelial morphology [DOI]
- 13 Effect of biomass concentration and mycelial morphology on fermentation broth rheology [DOI]
- 14 Suitability of the shaking flask for oxygen supply to microbiological cultures [DOI]
- 15 An inoculum technique for the production of fungal pellets [DOI]
- 16 Dependence of morphology on agitation intensity in fed‐batch cultures of Aspergillus oryzae and its implications for recombinant protein production [DOI]
- 17 Protease secretion in glucoamylase producer Aspergillus niger cultures: fungal morphology and inoculum effects [DOI]
- 18 Effect of mechanical agitation on the production of cellulases by Trichoderma reesei RUT-C30 in a draft-tube airlift bioreactor [DOI]
- 19 Influence of culture media and environmental factors on mycelial growth and sporulation of Lasiodiplodia theobromae (Pat.) Griffon and Maubl.
- 20 Insights into Fungal Morphogenesis and Immune Evasion [DOI]
- 21 The Morphology of Filamentous Fungi [DOI]
- 22 Morphological measurements on filamentous microorganisms by fully automatic image analysis [DOI]
- 23 Effects of agitation intensity on mycelial morphology and protein production in chemostat cultures of recombinant Aspergillus oryzae [DOI]
- 24 Relationship between morphology and citric acid production in submerged Aspergillus niger fermentations [DOI]
- 25 Influence of Morphology on Product Formation in Aspergillusawamori during Submerged Fermentations [DOI]
- 26 A STANDARD INOCULUM FOR CITRIC ACID PRODUCTION IN SUBMERGED CULTURE [DOI]
- 27 Effects of Increased Impeller Power in a Production‐Scale Aspergillus oryzae Fermentation [DOI]
- 28 Purification and some properties of a novel raw starch-digesting amylase fromAspergillus carbonarius [DOI]
- 29 Changes in membrane fluidity modulate heat shock gene expression and produced attenuated strains in the dimorphic fungus Histoplasma capsulatum.
- 30 Influence of fermentation conditions and scale on the submerged fermentation of Aspergillus awamori [DOI]
- 31 Effect of Temperature, Light and Media on Growth, Sporulation, Formation of Pigments and Pycnidia of Botryodiplodia theobromae Pat [DOI]
- 32 Correlation of Aspergillus niger broth rheological properties with biomass concentration and the shape of mycelial aggregates [DOI]
- 33 Influence of the process parameters on the morphology and enzyme production of Aspergilli [DOI]
- 34 Influence of reactor systems on the morphology of Aspergillus awamori. Application of neural network and cluster analysis for characterization of fungal morphology [DOI]
- 35 [A survey of temperature and pH effect on colonial growth of Botryodiplodia theobromae RC1].
- 36 Characterization of morphological forms of Aspergillus carbonarius and the effect of inoculum size on raw starch digesting amylase (RSDA) production [DOI]
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