Fungal Flora and Ochratoxin A Associated with Coffee in Cameroon
N. D. Nganou, N. Durand, N. L. Tatsadjieu, I. Métayer, D. Montet, C. M. F. Mbofung
Microbiology Research Journal International · pp. 1–17 · Published 5 Oct 2013
10.9734/BMRJ/2014/4284Abstract
Aims: 104 samples were collected from the west region and the coastal plain of Cameroon during two coffee campaigns, 2009 and 2010. Two coffee processes were evaluated (wet and dry processes) at different stages from harvesting to storage. Study Design: Food contaminants. Place and Duration of Study: Food Microbiology Laboratory, Department of Food Science and Nutrition (ENSAI) University of Ngaoundere; UMR 95 Qualisud, CIRAD of Montpellier, between May 2009 and September 2012. Methodology: Fungi profile was evaluated by direct plating techniques and identified using morphological and molecular tools. OTA levels were analyzed using HPLC technique after extraction and filtration using an immunoaffinity column. Results: Results obtained revealed an overall percentage of fungal contamination between 60-92% in 2009 and 70-90% in 2010. There was no ecological difference in the composition of ochratoxigenic species present in five sites. Coffee beans sampled in 2009 had a colonization incidence of 18-40% A. carbonarius, 12-22% A. niger, 3-15% A. ochraceus while those of 2010 had a colonization incidence of 15-30% A. carbonarius, 35-40% A. niger, and 2-7% A. ochraceus. Fungal diversity was not correlated with the geographical origin, coffee cultivar and processing method. There was no difference between the processes studied in terms of occurrence of ochratoxigenic fungi. OTA levels were mostly below the recommended standards although some isolated cases of extreme contamination were observed in 2009. A higher level of OTA was detected in the presence of A. niger, A. carbonarius and A. ochraceus than when only A. niger was present. Conclusion: The important fungi with the potential to produce OTA in Cameroonian coffee beans are A. carbonarius and A. niger. These two species were predominant on each type of coffee beans. It was also observed that once a toxigenic strain was isolated from a coffee sample, the sample contained OTA.
References (31)
- 1 Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [DOI]
- 2 Taxonomic Note: A Place for DNA-DNA Reassociation and 16S rRNA Sequence Analysis in the Present Species Definition in Bacteriology [DOI]
- 3 Introduction to food-borne fungi.
- 4 Discovery and Classification of Ecological Diversity in the Bacterial World: The Role of DNA Sequence Data [DOI]
- 5 Dichloran-glycerol medium for enumeration of xerophilic fungi from low-moisture foods [DOI]
- 6 Biodiversity of Aspergillus species in some important agricultural products [DOI]
- 7 The source of ochratoxin A in Brazilian coffee and its formation in relation to processing methods [DOI]
- 8 New ochratoxin A producing species of Aspergillus section Circumdati
- 9 Microbial diversity during maturation and natural processing of coffee cherries of Coffea arabica in Brazil [DOI]
- 10 Isolation, identification and toxigenic potential of ochratoxin A-producing Aspergillus species from coffee beans grown in two regions of Thailand [DOI]
- 11 Production of ochratoxin A by Aspergillus carbonarius on coffee cherries [DOI]
- 12 Old and new concepts of species differentiation inAspergillus [DOI]
- 13 Screening on the Occurrence of Ochratoxin A in Green Coffee Beans of Different Origins and Types [DOI]
- 14 Survey of aflatoxin B1and ochratoxin A in commercial green coffee beans by high‐performance liquid chromatography linked with immunoaffinity chromatography [DOI]
- 15 Sequencing and Analysis of Fungal rRNA Operons for Development of Broad-Range Fungal PCR Assays [DOI]
- 16 The effects of yeasts involved in the fermentation of Coffea arabica in East Africa on growth and ochratoxin A (OTA) production by Aspergillus ochraceus [DOI]
- 17 Behavior of Ochratoxin A during Green Coffee Roasting and Soluble Coffee Manufacture [DOI]
- 18 Occurrence of Ochratoxigenic Fungi and Ochratoxin A in Green Coffee from Different Origins [DOI]
- 19 Ochratoxin A-producing Aspergilli in Vietnamese green coffee beans [DOI]
- 20 Incidence of microflora and of ochratoxin A in green coffee beans (Coffea arabica) [DOI]
- 21 Aspergillus westerdijkiae polyketide synthase gene “aoks1” is involved in the biosynthesis of ochratoxin A [DOI]
- 22 Detection and quantification of Aspergillus westerdijkiae in coffee beans based on selective amplification of β-tubulin gene by using real-time PCR [DOI]
- 23 An update on ochratoxigenic fungi and ochratoxin A in coffee [DOI]
- 24 Molecular approach to the characterisation of fungal communities: methods for DNA extraction, PCR amplification and DGGE analysis of painted art objects [DOI]
- 25 Evaluation of PCR-DGGE methodology to monitor fungal communities on grapes [DOI]
- 26 Determination of fruit origin by using 28S rDNA fingerprinting of fungi communities by PCR-DGGE: an application to Physalis fruits from Egypt, Uganda and Colombia [DOI]
- 27 PCR method for the detection of potential ochratoxin-producing Aspergillus species in coffee beans [DOI]
- 28 Ochratoxin A contamination of coffee batches from Kenya in relation to cultivation methods and post-harvest processing treatments [DOI]
- 29 Inhibition of Species of the Aspergillus Section Nigri and Ochratoxin A Production in Grapes by Fusapyrone [DOI]
- 30 Determination of ochratoxin A in roasted coffee in Bangkok market.
- 31 Ocorrência e controle da microflora associada aos frutos de café (Coffea arabica L.) provenientes de diferentes localidades do estado de Minas Gerais
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