In vitro Antioxidant Properties of Various Extracts of Andrographis Echioides

Abstract

Background: Andrographis  echioides is a medicinal herb, used in the treatment of various diseases.  It has potential antioxidant properties.  The  present study plan is to find a novel herbal antidote for oxidative stress from Andrographis echioides. Objective: The aim of the current study is to analyse the in vitro antioxidant property of aqueous, ethanolic and chloroformic extracts of Andrographis echioides. Materials and Methods: DPPH free radical scavenging assay was performed to evaluate the antioxidant potential of Andrographis echioides. Experiments were carried out in triplicates and percentage inhibition of DPPH radical scavenging activity was calculated. The data was analysed statistically and the level of significance was considered at the level of p<0.05. Results: There is a dose dependent increase in the percentage of inhibition of DPPH free radical by the extracts. All the three extracts (aqueous, ethanolic and chloroformic extracts) of Andrographis  echioides showed significant increase in the antioxidant property with  concentration ranging from 100-500 µg.    Conclusion: The study concluded that different extracts of Andrographis echioides showed effective antioxidant properties and it could protect the biological system against oxidative stress including ageing, cancer, diabetes and cardiovascular disorders.