In silico Analysis of Transcription Factor Binding Sites and Impact of Defense Responsive Phytohormones in OsPR1a

Abstract

A variety of biotic and abiotic stress conditions result in the production and accumulation of pathogenesis related (PR) proteins in plant. Transcriptional regulation of PR genes plays a vital role in defense response in plant. In rice, the role of the PR1 gene in defense response have been studied, but critical examination of the OsPR1a gene after a treatment with defense responsive phytohormones and their regulation via promoter analysis have not been examined indepth. Several signalling mechanisms are involved in the induction and repression of defense genes, which are mediated by salicylic acid (SA) and jasmonic acid (JA). Expression profiling was carried out to determine the effects of phytohormones, salicylic acid (SA), and jasmonic acid (JA) at 12 h, 24 h, and 48 h after treatment. Expression profiling indicates cumulative upregulation of OsPR1a gene at 12 h after SA and JA treatments, whereas it downregulates at 24 h and 48 h after JA treatment. We also performed a comprehensive in silico analysis of the promoter region of OsPR1a gene to predict how the transcription factor binding site (TFBS) regulate its expression.