Variation Amount of Sperm Cells in the First Generation of Nontransgenic and Transgenic Mutiara Catfish Hybrid

Abstract

One of the factors that influence the success of catfish spawning, including male broodstock gonad fertility determine differences in the number of the sperm produced. The use of transgenesis technology (insertion of the exogenous growth hormone gene) in catfish shows an influence especially multiplication of the sperm counts. The purpose of this study was to compare the variations in the number of sperm cells of the male transgenic Mutiara catfish (comprising the African catfish growth hormone gene) with nontransgenic Mutiara. These research was conducted at the Fish Hatchery, Fisheries Biotechnology Laboratory, and Central Laboratory of Padjadjaran University. The experimental method was used completely randomized design and analyzed quantitatively and qualitatively. The male catfish broodstock were used as treatments consists of the first generation transgenic Mutiara hybrids with nontransgenic Mutiara (G1_MT-MNT), transgenic Mutiara hybrids with Sangkuriang (G1_MTS), and Sangkuriang (G1_S). The sperm from testes of each broodstock were  used  as  replications for sperm count test parameters and motility (n = 5). Qualitative analysis was used for the identification parameters of the transgenic male broodstock using the Polymerase Chain Reaction (PCR) and sperm volume. Quantitative analysis uses the Sigma Plot 12.0 program for parameters for sperm cell count and sperm motility. The results showed that the highest number of sperm (6.06 x 106 cells) and the highest motility (score 4.2) were found in the broodstock of the G1_MT-MNT male catfish.