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Ibnu Dwi Buwono

Publications (12)

Evaluation of the Effectiveness of Garlic Allium sativum Extract against Argulus sp. Infection and Hematological Responses in Goldfish Carassius auratus

Ibnu Dwi Buwono, Miqdad Robbani, Yuniar Mulyani & Roffi Grandiosa Herman · Asian Journal of Biochemistry, Genetics and Molecular Biology · 2026

Background: Infection by Argulus sp. is a significant issue in the ornamental fish industry, particularly for goldfish (C. auratus) because it can lead to tissue damage, haemorrhaging, stress, reduced appetite, secondary infections, and mortality. Current treatments for fish dise...

Open access Research Article 10.9734/ajbgmb/2026/v18i7553

Ovaprim Dosage Optimization for Spawning Induction of G6 Transgenic Mutiara Catfish

Ibnu Dwi Buwono, Indah Permata Juwita Putri, Roffi Grandiosa Herman & Yuniar Mulyani · Asian Journal of Biotechnology and Genetic Engineering · 2026

Background: Optimization of Ovaprim dosage is necessary to improve spawning success in sixth-generation (G6) transgenic Mutiara catfish carrying the Clarias gariepinus growth hormone (CgGH) gene, whose reproductive performance has not yet been comprehensively evaluated despite st...

Open access Research Article 10.9734/ajbge/2026/v9i2186

Effect of Tryptophan Supplementation in Commercial Feed on the Growth Performance of Mutiara Catfish Juveniles (Clarias gariepinus)

Ibnu Dwi Buwono, Ismi Nabila Putri, Iskandar Iskandar & Roffi Grandiosa Herman · Asian Journal of Biotechnology and Genetic Engineering · 2026

Background: Fish feed requires essential amino acids for optimal protein synthesis, and tryptophan supplementation at appropriate levels can improve growth performance, feed efficiency, and stress tolerance in juvenile fish while excessive or deficient levels can impair growth an...

Open access Research Article 10.9734/ajbge/2026/v9i1184

Genetic Polymorphism and Relationships in Hybrid Crosses of Butterfly Koi (Cyprinus carpio koi) and Comet Goldfish (Carassius auratus) Using RAPD Markers

Ibnu Dwi Buwono, Ujang Subhan & Fitri Awaliyah · Asian Journal of Biology · 2025

Cross-breeding of ornamental broodstock with close genetic relatives is one of the causes of the reduction of variations in the colour and body shape of fish offspring. The aim of this study is to analyse polymorphism in hybrid fish from butterfly female or male koi (bFK, bMK) an...

Open access Research Article 10.9734/ajob/2025/v21i12600

Gonado Somatic Index in G5 Transgenic Mutiara Catfish (Clarias gariepinus) at Different Temperatures

Ibnu Dwi Buwono, Iskandar, Ine Maulina & Ulfah Novia Suhendar · Asian Journal of Biotechnology and Genetic Engineering · 2024

Temperature is one of the factors that can affect the maturity of the gonads in fish. This study aims to find out what is the best temperature for maturation of broodstock to prepare G5 transgenic mutiara catfish broodstock that are ready to hatch based on gonado somatic index (G...

Open access Research Article

Variation Amount of Sperm Cells in the First Generation of Nontransgenic and Transgenic Mutiara Catfish Hybrid

Ibnu Dwi Buwono, Yuli Andriani, Walim Lili & Paksi Setyo Nugroho · Asian Journal of Biotechnology and Bioresource Technology · 2019

One of the factors that influence the success of catfish spawning, including male broodstock gonad fertility determine differences in the number of the sperm produced. The use of transgenesis technology (insertion of the exogenous growth hormone gene) in catfish shows an influenc...

Open access Research Article 10.9734/ajb2t/2019/v5i330060

Enhancing Colour Brightness in Barbir Fish (Puntius conchonius) Trough Dietary Supplementation with Pumpkin (Cucurbita moschata) Flour

Dinda Tazkia Putri, Ibnu Dwi Buwono, Kiki Haetami & Roffi Grandiosa · Asian Journal of Biotechnology and Genetic Engineering · 2025

This study aimed to evaluate the effect of adding pumpkin (Cucurbita moschata) flour to commercial feed on the colour brightness of barbir fish (Puntius conchonius), as well as to determine the optimal supplementation dose for maximum colour enhancement. The experiment was conduc...

Open access Research Article 10.9734/ajbge/2025/v8i1161

The Maturity of Female Gonads of G2 Transgenic Mutiara Catfish (Clarias sp.)

Ayuniar Puteri, Ibnu Dwi Buwono, Roffi Grandiosa Herman & . Iskandar · Asian Journal of Fisheries and Aquatic Research · 2021

The G2 trangsenic Mutiara catfish (Clarias sp.) (MTG) is a Mutiara catfish that is inserted with the CgGH gene (Clarias gariepinus Growth Hormone) through the transgenesis. The effect of transgenesis stimulates gonad growth of G2 transgenic Mutiara catfish (Clarias sp.) faster th...

Open access Research Article 10.9734/ajfar/2021/v12i330234

Genetic Kinship of Nilem Fish Strains (Osteochillus hasselti)

Yuli Andriani, Ibnu Dwi Buwono, Ujang Subhan & Arini Mandhasia · Asian Journal of Biochemistry, Genetics and Molecular Biology · 2020

The research aims to analyze the kinship of nilem fish strains using RAPD (Random Amplified Polymorphic DNA). This research used exploratory method with descriptive analysis. The primers used in the RAPD method are OPA-02 (5'-TGCCGAGCTG-3 '), OPA-03 (5'-AGTCAGCCAC-'3), OPA-05 (5'...

Open access Research Article 10.9734/ajbgmb/2020/v3i130077

Water Quality in the Cultivation of Catfish (Clarias gariepinus) and Nile Tilapia (Oreochromis niloticus) in the Aquaponic Biofloc System

Irfan Zidni, Iskandar ., Ibnu Dwi Buwono & Benedikta Prasiwi Mahargyani · Asian Journal of Fisheries and Aquatic Research · 2019

Aims: Water quality is one of them being an important role in terms of cultivation. Good water quality can support growth productivity in aquaculture. This is also of great concern when the rest of fish metabolism results that can contaminate fish contained in the maintenance med...

Open access Research Article 10.9734/ajfar/2019/v4i230048

Characteristics of Bacillus CgM22 and the Effect of Induction on the Growth Rate in Carp (Cyprinus carpio)

. Aisyah, Kiki Haetami, Yuniar Mulyani, . Iskandar, Ibnu Dwi Buwono & Roffi Grandiosa · Asian Journal of Fisheries and Aquatic Research · 2022

Bacillus is a potential probiotic candidate which is currently widely applied in aquaculture. Bacillus can be found in various hosts, one of which is found in the intestines of fish. This study contains the genus Bacillus subtilis intestinal fish Bacillus CgM22. The steps taken w...

Open access Research Article 10.9734/ajfar/2022/v18i430450