Anti-Inflammatory and Anti-cancer Activities of Leaf Extracts from Three Datura Species Found in Mali
Issiaka Togola, Mamadou Abdoulaye Konaré, Cheickna Cissé, Nouhoum Diarra
Journal of Applied Life Sciences International · pp. 88–97 · Published 16 Dec 2023
10.9734/jalsi/2023/v26i6630Abstract
The anti-inflammatory potential was estimated using cancer cells, by quantifying nitrites (NO) based on colorimetric method (Griess reaction) and the cell viability based on the WST-1 assay. The antiproliferative activity was assessed on cell lines (HeLa and MCF-7) using the Cell Titer Glo® assay. A phytochemical screening was conducted to identify the main groups of bioactive compounds present in these extracts. The phytochemical analysis revealed that leaf extracts from all three Datura species are sources of various secondary metabolites, including alkaloids, flavonoids and coumarins. The highest anti-inflammatory power was recorded with D. stramonium and D. innoxia extracts. with a concentration of 10 µg/mL of each extract, murine macrophages have normally grown, with NO inhibition rates of 30.05 ±3.11% and 25.70 ±2.04% for D. innoxia and D. stramonium respectively. The anticancer activity was more pronounced with D. stramonium extracts, which showed 13.33±3.05% of viability rate on the HeLa cell line at 50 µg/mL. In contrast, at the same concentration, the MCF-7 cell line was more sensitive to D. innoxia extracts, with a viability rate of 42.67±2.52%. The results of that study showed that three Datura species investigated have immunostimulant potential which could them to be useful in the treatment of breast and cervical cancers.
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Cited by 12
Mamadou A. Konaré, Cheickna Cissé, Issiaka Togola, Moussa Fomba · Annual Research & Review in Biology · 2024
Mohammad Attaullah, Hussain Ul Haq, Abdullah Khan · Current Pharmaceutical Design · 2025
Cheickna Cissé, Mamadou A. Konaré, Mariam Samaké · Specialty journal of Pharmacognosy, Phytochemistry, and Biotechnology · 2024
Cheickna Cissé, Mamadou A. Konaré, Mariam Samaké · International Journal of Pharmaceutical Research and Allied Sciences · 2024
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