Ameliorative Effect of Vitamin E on Paraquat Induced Haematological Disorder in Male Albino Rats

Abstract

Paraquat is a controversial herbicide that can increase reactive oxygen species levels by undergoing redox cycling and producing reactive oxygen species such superoxide anion. Vitamin E is a fat soluble vitamin that modulates oxidation processes in the body due to its particular antioxidant activity. It is a powerful chain-breaking antioxidant that limits the synthesis of reactive oxygen species molecules. The goal of the study was to see if vitamin E had a short-term therapeutic impact on paraquat-induced male albino rats. For the experiment, 200 male albino rats were employed. The 200 rats were separated into four primary groups (A, B, C, and D), each of which included 50 rats and was then subdivided into two subgroups, each with 25 rats. The "A" group was not induced paraquat, but the "B," "C," and "D" groups were induced 0.02g, 0.04g, and 0.06g of paraquat, respectively. The "A" group was divided into two subgroups: "A0" and "AVE," which represented the subgroups that were not given Vit E and those who were given Vit E       (500 mg) respectively. This design was similar for groups "B," "C," and "D." The frequency of paraquat introduction was fortnightly for three months, then followed by vitamin E treatment weekly for two month. After the treatment with Vit E, the rats were sacrificed and blood was taken to analyze several hematological parameters (Hemoglobin concentration [Hb], Packed Cell volume [PCV], Total White blood cell count [T-WBC], Neutrophils and Lymphocytes). These hematological parameters differed significantly across the "Ao," "Bo," "Co," and "Do" groups, at p-value < 0.05. This was equally true for the "Ave," "Bve," "Cve," and "Dve," groups at p-value < 0.05. The findings also revealed that intra-group comparisons of Hb, PCV levels between B0 vs Bve, C0 vs Cve, and D0 vs Dve were significant different, p-value<0.05 whereas WBC, neutrophils and  lymphocytes were not p-value>0.05. This study has shown that PQ insult on anaemia indicators in rats can be corrected by a weekly administration of the vitamin.