Ameliorative Effect of Ethanolic Fruit Extract of Perssea americana and Prunus dulcis on Caffein-Testicular Damage in Male Wistar Albino Rats

Abstract

This study investigated the effect if ethanolic fruit extracts of Perssea americana and Prunus dulcis on caffeine-testicular damage in male albino rats. Sixty rats weighing 120 and 170 g were used for this study. The rats were separated into 12 groups of five rats per group. Group 1 served as normal control, group 2 received 200mg/kg of caffeine, serving as negative control while group 3 served as positive control. Rats in group 4-6 were orally administered 200mg/kg caffeine + ethanolic 100, 200, and 400mg/kg of Prunus dulcis, group 7-12 received 200mg/kg caffeine + ethanolic 100, 200, and 400mg/kg, and Caffeine + ethanolic 100mg/kg Persea americana +100mg/kg Prunus dulcis fruit extract for 7, 14, and 21 days. Blood sample was collected from the tail of each rats at 7 days interval after treatment, for biochemical assays while one rats in each group was sacrificed and the testis was harvested for histological analysis. The plasma luteinizing hormone level of the negative control for 7, 14, and 21 days were 1.62 ± 0.01IU/ML, 1.71 ± 0.02IU/ML, and 1.05 ± 0.09IU/ML respectively. The plasma luteinizing hormone level of group 6 treated with 400mg/kg of Prunus dulcis fruit extract for 7, 14, and 21 days were 3.06. ± 0.05IU/ML, 3.41 ± 0.05IU/ML, and 3.63 ± 0.01IU/ML respectively, were significantly different from group 2 while those of group 9 treated with 400mg/kg of Persea americana fruit extract for 7, 14, and 21 days were 3.51 ± 0.05IU/ML, 3.82 ± 0.05IU/ML, and 3.90 ± 0.02IU/ML. Regeneration of damage testicular tissues occurred after treatment. The significant increases observed on the hormonal profile and regeneration of damaged testicular tissues of the extracts treated rats indicated testiculo-curative effect of P. dulcis and P. americana fruit on caffeine-induced testicular damage in rats.