The Screening of EST-SSR Markers Associated with Peel Color in Tomato Fruit

Abstract

Aims: The color of tomato fruit is an important property in improving of tomato breed. So we screened the differences of yellow tomato peel and transparent tomato peel using EST-SSR markers, and it is in favour of the research of the tomato fruit color and tomato breeding. Study Design: Primers were designed from ESTs in which the repeat bases of SSR were more than 18bp, then tested in yellow peel and transparent peel of gene pools built by F2 from E-95F8(♀) and Zhonghuajufeng8(♂). Place and Duration of Study: College of Biological Science and Technology, between February 2015 and March 2016. Methodology: The yellow peel gene pool and the transparent peel gene pool were built according to the trait segregation group of F2, and then screened the differential bands by the primers. In the end, the differential bands were tested in the segregation group of F2. Results: 250 primer pairs were designed. One (HML52) of 250 primer pairs had diversity between yellow peel and transparent peel of gene pools built by F2 from E-95F8(♀) and Zhonghuajufeng8(♂). In the end, the primer pair of HML52 passed test, and genetic distance was 7.04 cM. Conclusion: The primer pair of HML52 of EST-SSR marker associated with peel color in tomato was found in this study, and the genetic distance of the primer pair was 7.04 cM.