Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

V. N. Pishchik; N. I. Vorobyev; Yu. V. Ostankova; A. V. Semenov; A. Totolian Areg; A. A. Popov; Y. V. Khomyakov; O. R. Udalova; D. V. Shibanov; V. E. Vertebny; V. I. Dubovitskaya; O. V. Sviridova; O. S. Walsh; S. Shafian

doi:10.9734/IJPSS/2018/41148

Research Article Open access CC BY 4.0

Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer

V. N. Pishchik, N. I. Vorobyev, Yu. V. Ostankova, A. V. Semenov, A. Totolian Areg, A. A. Popov, Y. V. Khomyakov, O. R. Udalova, D. V. Shibanov, V. E. Vertebny, V. I. Dubovitskaya, O. V. Sviridova, O. S. Walsh, S. Shafian

International Journal of Plant & Soil Science · pp. 1–12 · Published 12 May 2018

10.9734/IJPSS/2018/41148

Abstract

In this study we investigated the mechanisms of action and effect of plant growth promoting rhizobacteria (PGPR) Bacillus subtilis No.2 when utilized alone and in conjunction with a humic fertilizer (HF). Different mechanisms of action of B. subtilis No.2 and HF Stimulife on tomato plants were identified in pot experiments under controlled conditions. PGPR B. subtilis No.2 was identified by using the16s rRNA gene sequence method. We applied factor analyses to evaluate differences in the responses of plants to the individual effects of B. subtilis No.2 and HF when they were used together. Auxin-producing B. subtilis No.2 enhanced tomato yield by increased average number of fruits per plant. Humic fertilizer Stimulife, which also contains auxins, improved tomato yield by increasing average fruit weight. As shown by factor analysis, the impact of weight coefficients for plant responses (across tomato varieties) to B. subtilis No.2 and Stimulife were 0.54 and 0.28, respectively, indicating a greater response to B. subtilis No.2 than to HF Stimulife. Combined use of Stimulife and B. subtilis No.2 had a positive impact on tomato yield, increasing fruit yield by 25-29%. Tomato fruit quality was improved by increasing the amounts of dry matter, carbohydrates, sugar-index acid, and ascorbic acid. Results suggest that HF Stimulife and B. subtilis No.2could be successfully used to enhance tomato plant growth and yield under controlled conditions. We hypothesize that, along with direct impact, HF may also indirectly affect plants by stimulating PGPR.

PGPR Bacillus subtilis No.2 humic fertilizer tomato physiological and biochemical characteristics

Cited by 16

Microbial Inoculation Strategies for Optimal Cherry Tomato Production

T. D. Silva, R. Ferrarezi, Priscila Jane Romano Gonçalves Selari · Physiologia Plantarum : An International Journal for Plant Biology · 2025

Changes in Yield and Antioxidant Enzyme Content of Tomato Plant Depending on the Application of Microorganism Injected Tuff Material

Adem Güneş, Leyla Tarhan, Bahar Soğutmaz Özdemir · Communications in Soil Science and Plant Analysis · 2022

Promoción del crecimiento de tomate saladette con Bacillus cereus y estiércol solarizado en invernadero

Alfonso Andrade-Sifuentes, M. Fortis-Hernández, P. Preciado-Rangel · Revista Mexicana de Ciencias Agrícolas · 2022

Effects of Plant-Growth-Promoting Rhizobacteria (PGPR) and Cyanobacteria on Botanical Characteristics of Tomato (Solanum lycopersicon L.) Plants

Ebtesam A. Gashash, Nahid A. Osman, A. A. Alsahli · Plants · 2022

Water Stress Alleviation Effects of Biostimulants on Greenhouse-Grown Tomato Fruit

Â. Fernandes, Christina Chaski, Carla Pereira · Horticulturae · 2022

Improving the growth, P uptake and quality characteristics of ‘Lollo Rosso’ lettuce in the nutrient solution by Bacillus subtilis in different phosphorus concentrations

E. Shabani · Journal of plant nutrition · 2022

Epiphytic PGPB Bacillus megaterium AFI1 and Paenibacillus nicotianae AFI2 Improve Wheat Growth and Antioxidant Status under Ni Stress

V. Pishchik, P. S. Filippova, G. Mirskaya · Plants · 2021

Bacillus velezensis 83 increases productivity and quality of tomato (Solanum lycopersicum L.): Pre and postharvest assessment

K. Balderas-Ruíz, C. Gómez-Guerrero, M. Trujillo-Roldán · Current Research in Microbial Sciences · 2021

Case study: Planting methods and beneficial substrate microbes effect on the growth of vegetated roof plants in Finland

Long Xie, Susanna Lehvävirta, J. Valkonen · 2020

Extending thermotolerance to tomato seedlings by inoculation with SA1 isolate of Bacillus cereus and comparison with exogenous humic acid application

M. Khan, S. Asaf, A. Khan · PLoS ONE · 2020

Access this article

View at publisher

Cite this article

V. N. Pishchik, N. I. Vorobyev, Yu. V. Ostankova, A. V. Semenov, A. Totolian Areg, A. A. Popov, Y. V. Khomyakov, O. R. Udalova, D. V. Shibanov, V. E. Vertebny, V. I. Dubovitskaya, O. V. Sviridova, O. S. Walsh, S. Shafian (2018). Impact of Bacillus subtilis on Tomato Plants Growth and Some Biochemical Characteristics under Combined Application with Humic Fertilizer. International Journal of Plant & Soil Science , 1–12. https://doi.org/10.9734/IJPSS/2018/41148

Affiliations

V. N. Pishchik — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
N. I. Vorobyev — All-Russian Research Institute for Agricultural Microbiology of Russian Academy of Agricultural Science, St. Petersburg, Russia.
Yu. V. Ostankova — Research Institute Pasteur, Russia.
A. V. Semenov — Research Institute Pasteur, Russia.
A. Totolian Areg — Research Institute Pasteur, Russia.
A. A. Popov — All-Russian Research Institute for Agricultural Microbiology of Russian Academy of Agricultural Science, St. Petersburg, Russia.
Y. V. Khomyakov — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
O. R. Udalova — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
D. V. Shibanov — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
V. E. Vertebny — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
V. I. Dubovitskaya — Agrophysical Research Institute of Russian Agricultural Academy, St. Petersburg, Russia.
O. V. Sviridova — All-Russian Research Institute for Agricultural Microbiology of Russian Academy of Agricultural Science, St. Petersburg, Russia.
O. S. Walsh — Department of Plant Sciences, University of Idaho, Parma, ID, USA.
S. Shafian — Department of Plant Sciences, University of Idaho, Parma, ID, USA.

Metrics

0
Views: 0
Downloads: 16
Citations: 0
Outbound

Full metrics

Article metrics

Real usage data collected on this platform.

0

Page views

0

PDF downloads

0

Outbound clicks

16

Citations

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

No views recorded yet.

Traffic sources

Referring site, by host.

No traffic recorded yet.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.