Amelioratory Effect of Dorema glabrum on Diazinon-Induced Oxidative Stress in Rat Liver

Abstract

Introduction: A wide number of pesticides, including highly persistent organophosphorous compounds, such as diazinon (DZN) have deteriorating effect on fauna and flora by inducing oxidative stress. DZN induces cell damage by producing free radicals and reactive oxygen species. In addition to the antioxidant enzymes such as catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD) activities in the liver tissue, reactive oxygen species (ROS) have been employed in the toxicity of organophosphate insecticides (OPIs) and the level of lipid peroxidation was analyzed. The present study was designed to explore the ameliorative characteristics of D. glabrum against the subchronic impact of DZN on such oxidative damage markers as lipid peroxidation (LPO) and the antioxidant defense system (ADS) existing in the liver of male Wistar rats. Methods: Twenty-four adult male Wistar rats were used in this study. The rats randomly divided into four groups including a control group, and three experimental groups. Two of three experimental groups received different doses of D. glabrum (40 and 80 mg/kg) as pre-treatment for 21 days along with DZN (100 mg/kg) that injected intraperitoneally in the last day of D. glabrum usage, and one group received only DZN (100 mg/kg). Results: Compared with the control group, we noticed significantly high levels of LPO and the low antioxidant defenses, like free radical scavenging enzymes viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) in DZN-treated group. Considering the hepatic toxicity of DZN, evident changes were also noticed in endogenous antioxidant enzyme along with high LPO levels. In rats supplemented with D. glabrum as well as treated with DZN, hepatic specific marker enzymes were restored to normalcy which otherwise was lowered in the DZN-treated rats. The obtained results revealed that the oxidative stress of DZN-treated rats is diminished when D. glabrum is co-treated with DZN. This co-treatment may also act as a putative protective agent against DZN-induced liver tissue injury.