Quantitation of TLR-2 mRNA Expression in Bovine Mastitis Caused by E. coli

Abstract

Aims: This study aim was to assay the expression of an important TLR2 gene in naturally infected cows with sub-clinical and clinical mastitis caused by E. coli. Methodology: cDNA from E. coli infected samples were obtained from hind quarters of three animals with sub-clinical mastitis, three animals with clinical mastitis along with a healthy animal, were used for TLR2 expression analysis using commercial kit and oligo (dT) primers. Quantitative reverse transcription PCR (RT-qPCR) were standardized for the TLR2 and β-actin gene, genes expression level was analyzed by Illumina Eco® Q- RT PCR system. Results: The mRNA expression of TLR2 gene in animals sub-clinical mastitis was significant (P ≤ 0.01) higher (3.63 fold) than clinical case (1.89 fold) when compared to healthy bovine. Conclusion: The expression of TLR2 gene during early stage of infection was at high level, therefore most of the sub-clinical mastitis subsided by itself without precipitating into clinical mastitis. It is suggested that TLR2 may consider as candidate gene for mastitis screening in cattle population.