Tyrosinase Inhibition, Antioxidant and Antibacterial Activity of Commercial Daisy Extract (Bellis perennis)
Enida Karić, Emir Horozić, Saša Pilipović, Esmeralda Dautović, Merima Ibišević, Amra Džambić, Semir Čeliković, Arnela Halilčević
Journal of Pharmaceutical Research International · pp. 13–19 · Published 23 Mar 2023
10.9734/jpri/2023/v35i57325Abstract
Extracts obtained from plant material have widely applied in the chemical and pharmaceutical industries because they contain significant concentrations of biologically active substances. Commercial daisy extract (Bellis perennis) was used in this paper for in vitro testing of tyrosinase enzyme inhibition, and antioxidant and antimicrobial activity. Inhibition of the tyrosinase enzyme was determined by monitoring dopachrome formation at a wavelength of 492 nm. Antioxidant activity was tested using FRAP and DPPH methods, while antibacterial activity was tested by diffusion technique on reference strains from the ATCC collection. The results showed that daisy extract inhibits tyrosinase enzyme in a dose-dependent manner. The extract effectively neutralized DPPH radicals and also showed good reducing ability. Bacterial strains used for in vitro antimicrobial activity testing did not show sensitivity to the extract concentrations used in this study.
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