Micropropagation Via Embryogenesis Techniques under Commercial Scale of Vulnerable Agarwood (Aquilaria crassna Pierre ex Lecomte)

Abstract

Introduction: Agarwood is an important tree having valuable in medicine, pharmaceutical and cosmetic. Completely exploited by humans and driven to extinction. Reforestation of this precious tree species is necessary via seedlings production and supply to enlarge growing regions. Aim: Micropropagation via somatic embryogenesis of Golden Teak sourced from Myanma for regeneration and develoment of valuable tree species Methods: Using method cultivation of somatic embryos technique Results: Leaf and root explants were the well samples for somatic embryogenesis cultivation. MS medium is suitable for the process of culturing agarwood somatic embryos. Culturing young leaves to create embryogenic callus. Embryogenic callus is multiplied in liquid medium. The embryogenic callus cell suspension is spread on semi-solid medium; to regenerate the embryogenic callus with increased biomass differentiated into embryogenic cells. Embryogenic cells are cultured on regeneration medium after 120 days of shoot formation. Single shoots are used as raw materials for conservation and micropropagation. Conclusion: Conservation as this species is highly exploited for industrial uses. The report on agarwood regeneration for far micropropagation was set up.