Spectrophotometric and High Performance Liquid Chromatographic Determination of Carbamazepine in Tablets Dosage Form

Abstract

Today, millions of people suffer from epilepsy, one of the most common chronic neurological diseases worldwide. Carbamazepine is a first-line drug used in the treatment of epilepsy. High performance liquid chromatographic and spectrophotometric methods have been developed for the determination of carbamazepine in tablet dosage forms. UV spectrums were recorded in the wavelength range of 200-800 nm using methanol solvent, and the wavelength for determining carbamazepine was selected as 286 nm. LC analysis was performed using Agilent Extend-C18 column and mobile phase composed of KH2PO4 solution(pH: 3.5) and acetonitrile (40:60 v/v) at a flow rate of 1.2 mlmin-1. These analytical methods were validated in agreement with the International Conference on Harmonization (ICH) guidelines using the following analytical parameters: specificity, linearity, precision, accuracy, detection and quantification limits, and robustnes. Analytical methods showed wonderful linearity (r2>0.999) in the concentration range of 5-25 μg mL-1 for boths methods. Precision (R.S.D%<1.17) and recevery for both methods was in the range of 99-101%, which shows accuracy of these methods. These proposed methods were found to be accurate, reliable, fast, simple, The F-test and t-test were used to perform statistical comparison of these methods, and the results of both analytical methods indicated no significant difference. As a result, the proposed methods can be used to analyze carbamazepine in pharmaceutical formulations.