Sodium Dodecyl Sulfate-cured Nalidixic Acid Resistant 85-kb Plasmid Salmonella gallinarum Immunoglobulin G Response in Brown Layer Hens
Ogochukwu P. Offor, Ngozi Offor, Damian C. Odimegwu, Wilfred I. Ugwuoke, Ebele B. Onuigbo
Asian Journal of Research in Animal and Veterinary Sciences · pp. 143–148 · Published 1 Aug 2022
10.9734/ajravs/2022/v5i2207Abstract
Aim: Fowl typhoid vaccination is a necessary complement to farm hygiene in reducing antimicrobial resistance caused by extensive prophylaxis of antibiotics in poultry. This study was undertaken to develop a vaccine candidate from a virulent strain by plasmid-curing. Place and Duration of Study: The research was carried out in the Department of Pharmaceutical Microbiology and Biotechnology, University of Nigeria Nsukka, for six months. Methodology: Thirty day-old pullets were divided into three groups of ten birds each. This comprised a negative control group (unvaccinated) (NEG), a live SG9R vaccine positive group (SG9R), and a nalidixic acid resistant plasmid-cured 85-kb plasmid Salmonella gallinarum (NAR). Plasmid curing of the virulent strain was done by incubating in sodium dodecyl sulfate (SDS) and loss of the 85-kb plasmid was identified and determined on agarose gel electrophoresis. Vaccination was done subcutaneously at 4 and 7 weeks of age, followed by challenge with the virulent S. gallinarum. IgG was measured using Enzyme-linked Immunosorbent Assay (ELISA). The scalability of the SDS-cured nalidixic acid resistant 85kb plasmid Salmonella gallinarum immunity was demonstrated by vaccinating layer birds and comparing the humoral immunity with that of a commercial fowl typhoid vaccine (SG9R). Results: There were higher IgG levels in the NAR group than the SG9R group. Protection was above 70 % in the vaccinated groups. Conclusion: The outcome of this present study shows that vaccination with viable cells of sodium dodecyl sulfate- cured nalidixic acid resistant 85-kb plasmid Salmonella gallinarum (NAR) provided layer birds with protective humoral immunity against subsequent challenge with the parent virulent strain containing the 85-kb plasmid.
References (14)
- 1 Rapid procedure for detection and isolation of large and small plasmids [DOI]
- 2 MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS [DOI]
- 3 Fowl typhoid and pullorum disease [DOI]
- 4 Pullorum disease and fowl typhoid—new thoughts on old diseases: a review [DOI]
- 5 Salmonella Pathogenicity and Host Adaptation in Chicken-Associated Serovars [DOI]
- 6 Immunity to salmonellosis [DOI]
- 7 Rapid Procedure for Detection and Isolation of Large and Small Plasmids [DOI]
- 8 Contribution of Salmonella gallinarum large plasmid toward virulence in fowl typhoid [DOI]
- 9 Identification of Salmonella gallinarum virulence genes in a chicken infection model using PCR-based signature-tagged mutagenesis [DOI]
- 10 Feeding an army: The metabolism of T cells in activation, anergy, and exhaustion [DOI]
- 11 Genetic resistance to Salmonella infection in domestic animals [DOI]
- 12 Plasmid curing in bacteria [DOI]
- 13 The Association Between a Large Molecular Mass Plasmid and Virulence in a Strain of Salmonella pullorum [DOI]
- 14 Immunity to experimental fowl typhoid in chickens induced by a virulence plasmid-cured derivative of Salmonella gallinarum [DOI]
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