Serological Detection of Cucumber Mosaic Virus and Capsicum Chlorosis Virus in Pepper (Capsicum annuum L.) Germplasm

Abstract

A survey was conducted on the basis of symptomatology for the screening of germplasm under insect proof glasshouse conditions in the Department of Vegetable Science, Dr. YSPUHF, Nauni, Solan to identify the sources of resistance in pepper crop. The most prominent symptoms were mosaic, mottling, leaf narrowing, stunted growth, mosaic with chlorotic lesions, formation of rings with necrotic lesions and mosaic with mottling. Forty-eight varieties or breeding lines were screened for the presence or absence of virus through DAS-ELISA. The study revealed that nineteen varieties or breeding lines tested positive and twenty-nine tested negative for virus infection. Sweet Banana variety recorded the least O.D. value followed by SB and UHF-Cap-30 whereas, UHF-Cap-22 was most susceptible variety with maximum O.D. value. Mixed infection of CMV and capsicum chlorosis orthotospovirus (CaCV) was recorded with six varieties or breeding lines during germplasm screening. This study signifies the presence of mixed infection with chlorotic lesions like symptoms and importance of DAS-ELISA in screening of pepper germplasm against CMV and CaCV.