Regeneration of Shoots from Callus of Ceropegia pusilla Wight and ARN

Abstract

This study was undertaken to evaluate the most suitable concentration of plant growth regulators and suitable explants for callus induction and subsequent organogenesis of an endangered medicinal plant Ceropegia pusilla. The best performance of callus induction and morphogenesis was found on MS medium supplemented with 6- benzylaminopurine (BA) and naphthalene acetic acid (NAA), from node and internode the maximum account of callus initiation was recorded on MS medium supplemented with BA+NAA (2.22µM + 5.37 µM) and the maximum % of callus induced on subculture is 5.47 ± 0.68. Rooting was best achieved on MS medium augmented with IBA (2.46µM). The maximum number of roots (4.72 ± 0.66 cm) and root length (4.8 ± 0.49 cm) were recorded). Plantlets regenerated in vitro with well-developed shoot and roots were successfully acclimatized in pots containing a mixture of decomposed coir waste, perlite and compost 1: 1:1 ratio and grown in a shade house with 81 ± 3.16 percent survival rates.