RP-HPLC Method Development and Validation for Determination of Tigecycline in Bulk and Pharmaceutical Dosage form
K. Bhavyasri, C. H. Mounika, Narmada Vallakeerthi, M. Sumakanth
Journal of Pharmaceutical Research International · pp. 273–282 · Published 3 Aug 2021
10.9734/jpri/2021/v33i39B32204Abstract
Aims: To develop and validate a new, simple, rapid, precise and accurate Reverse Phase High Performance Liquid Chromatographic (RP-HPLC) method for the quantitative determination of Tigecycline in bulk and pharmaceutical dosage form. Study Design: Place and Duration of the Study: RBVRR women's college of pharmacy, Barkatpura, Hyderabad, between june 2019 and july 2020. Methodology: The RP-HPLC method was developed on Sunsil C18 150 mm x 4.6mm x 5µ column using acetonitrile : water (pH maintained at 3.5 with acetic acid) [70:30] as mobile phase at flow rate 0.8 ml/min and UV detection at 250 nm. Results: Tigecycline exhibited linearity over the concentration range of 5-40 µg/mL (R2 > 0.999). The analytical method showed good precision with % RSD below 2. The method showed suitable accuracy and robustness. Conclusion: Validation of the developed method was done as per International Conference on Harmonization (ICH) Q2R1 guidelines.
Cited by 7
Khanderao R. Jadhav Khanderao R. Jadhav · Journal of Carcinogenesis · 2024
Rimadani Pratiwi, Putri Nur Azizah, Aliya Nur Hasanah · Microchemical Journal · 2024
Hani Mohammed Hafez, Sona Soliman Barghash, Marwa M. Soliman · F1000Research · 2023
Jyoti N. Mittha, Mallinath S. Kalshetti · Research Journal of Pharmacy and Technology · 2022
Bansi Rakholiya, Priyangi Shah, Yash Patel · Journal of AOAC International · 2023
Hani Mohammed Hafez, Sona Soliman Barghash, Marwa M. Soliman · F1000Research · 2023
Showing 6 of 7 known citations — external sources report more than can currently be individually listed.
Related research
- Antimicrobial Resistance Pattern of Multidrug Resistant Enterobacteriaceae (MDRE) Isolated from Clinical Samples with Special Reference to Carbapenemase Production and Susceptibility to Tigecycline — shares topic coverage
- Tigecycline Resistant Pattern among Carbapenem Resistant Gram-negative Bacilli: Prospective Cross-sectional Study — shares topic coverage
- Emergence of Third Generation Tetracyclines: New Magic Bullets to Tackle Antibiotic Resistance in the Post-antibiotic Era — shares topic coverage
- Study of Interaction between Tigecycline and Sulbactam — shares topic coverage
- Prevalence of Gram Negative Infections by Acinetobacter and Pseudomonas Severely Resistant to Antibiotic Susceptibility Based on Minimum Growth Inhibitor Concentration — shares topic coverage
Article metrics
Real usage data collected on this platform.
12
Page views
0
PDF downloads
0
Outbound clicks
7
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.