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Research Article Open access CC BY 4.0

Tigecycline Resistant Pattern among Carbapenem Resistant Gram-negative Bacilli: Prospective Cross-sectional Study

Jyoti, Chinmoy Sahu, Richa Sinha, Ashutosh Pathak, Gerlin Verghese

South Asian Journal of Research in Microbiology · pp. 21–29 · Published 26 Aug 2024

10.9734/sajrm/2024/v18i9385

Abstract

Introduction: Tigecycline is a unique tetracycline class of semi-synthetic, last-line broad spectrum antibiotic against multi-drug-resistant bacteria. However, recently, resistance to this antibiotic is on the rise. Aims: This study was conducted to determine the prevalence of tigecycline resistance amongst carbapenem-resistant Gram-negative bacilli (GNB) isolated from clinical samples (pus and sputum) as well as to evaluate their antimicrobial susceptibility pattern.  Study design:  Prospective cross sectional Place and Duration of Study: Department of Microbiology at Sanjay Gandhi Post Graduate Institute of Medical Sciences Lucknow, between January 2023 and December 2023. Methodology: Identification of GNB grown on culture was done by conventional biochemical tests and later validated by MALDI-TOF MS. The antimicrobial sensitivity testing of isolates was done using the E-test, and disk diffusion method. Minimum inhibitory concentration determination was done by Broth micro-dilution (BMD) method. Results: Amongst 8326 pus and respiratory samples, GNBs were recovered from 63.15% (5258/8326). Of 5258 GNB isolates, 50.74% (2668) were carbapenem-resistant, while 7.85% (413) demonstrated resistance to both tigecycline and carbapenem. Common isolates in this group were Klebsiella pneumoniae (37.04%), Acinetobacter spp. (25.18%), Enterobacter spp. (14.28%) and Escherichia coli (12.59%). BMD results demonstrated highest activity of tigecycline against carbapenem-resistant E. coli, followed by Citrobacter and Enterobacter. It works against resistant strains of Acinetobacter baumannii and K. pneumoniae as well, but in higher concentrations. Conclusion: High tigecycline resistance (one of the last-resort drugs) among carbapenem resistant GNB isolates is a matter of clinical concern, leaving physicians with limited options for treatment of such infections. Proper adherence to the policies of antimicrobial stewardship programs can reduce the emergence of resistance.

Antibiotic bacteria carbapenem-resistant gram-negative tigecycline

References (20)

  1. 1 Performance standards for antimicrobial susceptibility testing
  2. 2 Tetracycline Antibiotics: Mode of Action, Applications, Molecular Biology, and Epidemiology of Bacterial Resistance [DOI]
  3. 3 Antibacterial resistance worldwide: causes, challenges and responses [DOI]
  4. 4 A Brief History of the Antibiotic Era: Lessons Learned and Challenges for the Future [DOI]
  5. 5 Pharmacokinetics of Tigecycline after Single and Multiple Doses in Healthy Subjects [DOI]
  6. 6 Tigecycline antibacterial activity, clinical effectiveness, and mechanisms and epidemiology of resistance: narrative review [DOI]
  7. 7 In vitro antibacterial activities of tigecycline in combination with other antimicrobial agents determined by chequerboard and time-kill kinetic analysis [DOI]
  8. 8 Antimicrobial susceptibility of Gram-negative organisms isolated from patients hospitalised with pneumonia in US and European hospitals: Results from the SENTRY Antimicrobial Surveillance Program, 2009–2012 [DOI]
  9. 9 Global Assessment of the Activity of Tigecycline against Multidrug-Resistant Gram-Negative Pathogens between 2004 and 2014 as Part of the Tigecycline Evaluation and Surveillance Trial [DOI]
  10. 10 Tigecycline (Tygacil): The First in the Glycylcycline Class of Antibiotics [DOI]
  11. 11 Multidrug-resistant bacteria: what is the threat? [DOI]
  12. 12 Bacterial species identification using MALDI-TOF mass spectrometry and machine learning techniques: A large-scale benchmarking study [DOI]
  13. 13 Activity of Tigecycline in Combination with Colistin, Meropenem, Rifampin, or Gentamicin against KPC-Producing Enterobacteriaceae in a Murine Thigh Infection Model [DOI]
  14. 14 Surveillance of tigecycline activity tested against clinical isolates from a global (North America, Europe, Latin America and Asia-Pacific) collection (2016) [DOI]
  15. 15 Evaluation of tigecycline activity in clinical isolates among Indian medical centers [DOI]
  16. 16 Regional variations in multidrug resistance among Enterobacteriaceae in the USA and comparative activity of tigecycline, a new glycylcycline antimicrobial [DOI]
  17. 17 Resistance of Gram-negative Bacilli to Antimicrobials [DOI]
  18. 18 Tetracycline Antibiotics and Resistance [DOI]
  19. 19 Antimicrobial Susceptibility Testing [DOI]
  20. 20 Evolution and Mechanisms of Tigecycline Resistance in Escherichia coli

Cited by 4

Genetic characterization of tigecycline and carbapenem resistant Acinetobacter baumannii in a tertiary care hospital of Northern India

Jyoti Chaudhary, U. Ghoshal, A. Singh · BMC Microbiology · 2026

Molecular characterization and transmission pattern of tetracycline resistance determinants in tigecycline and carbapenem resistant Klebsiella pneumoniae isolates at a tertiary care hospital in India

J. Chaudhary, Richa Sinha, Irfan Hasan · Access Microbiology · 2026

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