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Research Article Open access CC BY 4.0

Quantitation of TLR-2 mRNA Expression in Bovine Mastitis Caused by E. coli

R. Lakshmi, K. K. Jayavardhanan

International Journal of Biochemistry Research & Review · pp. 1–5 · Published 14 Jun 2017

10.9734/IJBCRR/2017/33367

Abstract

Aims: This study aim was to assay the expression of an important TLR2 gene in naturally infected cows with sub-clinical and clinical mastitis caused by E. coli. Methodology: cDNA from E. coli infected samples were obtained from hind quarters of three animals with sub-clinical mastitis, three animals with clinical mastitis along with a healthy animal, were used for TLR2 expression analysis using commercial kit and oligo (dT) primers. Quantitative reverse transcription PCR (RT-qPCR) were standardized for the TLR2 and β-actin gene, genes expression level was analyzed by Illumina Eco® Q- RT PCR system. Results: The mRNA expression of TLR2 gene in animals sub-clinical mastitis was significant (P ≤ 0.01) higher (3.63 fold) than clinical case (1.89 fold) when compared to healthy bovine. Conclusion: The expression of TLR2 gene during early stage of infection was at high level, therefore most of the sub-clinical mastitis subsided by itself without precipitating into clinical mastitis. It is suggested that TLR2 may consider as candidate gene for mastitis screening in cattle population.

TLR2 expression mastitis E. coli

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