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Research Article Open access CC BY 4.0

In vitro Antibacterial Activity of Ocimum suave Essential Oils against Uropathogens Isolated from Patients in Selected Hospitals in Bushenyi District, Uganda

Julius Tibyangye, Matilda Angela Okech, Josephat Maniga Nyabayo, Jessica Lukanga Nakavuma

Microbiology Research Journal International · pp. 489–498 · Published 8 May 2015

10.9734/BMRJ/2015/17526

Abstract

Aims: To determine antibacterial activity of Ocimum suave essential oils against bacterial uropathogens. Study Design: A cross sectional and experimental study. Place and Duration of Study: Six selected hospitals in Bushenyi District, Uganda between June 2012 and July 2013. Methodology: Clean catch midstream urine samples were collected and inoculated on Cystine Lysine Electrolyte Deficient (CLED) agar. The plates were incubated at 37°C for 24hrs to 48hrs. The O. suaveessential oils were extracted by hydrodistillation of leaves for 4hrs using a Clevenger apparatus. The oil was collected and dried over anhydrous sodium sulphate (Na2SO4) and kept at 4°C till further use. The antimicrobial activity of O. suave essential oils against isolates was determined by agar well method. The MIC of O. suave essential oil extract was carried out by micro-broth dilution method. Results: Of the three hundred (300) midstream urine samples collected, 67(22.33%) had significant bacterial growth. Escherichia coli is the most common isolate (61.19%, n = 41). The essential oil from O. suave showed activity against isolates of E. coli, K. pneumoniae, S. aureus, E. feacalis, M. morganii, Citrobacter species, Enterobacter species and P. aeruginosa with mean zone of inhibition (ZI) ranging from 10-22 mm. The essential oils had no inhibitory activity on Acinetobacter species. The minimum inhibitory concentration (MIC) for O. suave essential oils ranged from 0.78 to 22 µg/ml. This study showed that O. suave essential oils had MIC value of 0.78 µg/ml against S. aureus and MIC values ranging from 3 to 22 µg/ml against the other tested isolates. Conclusion: The most common uropathogen was E. coli (61.19% n = 41). O. suave essential oils exhibited antibacterial activity against majority of the uropathogens, except Acinetobacter species, mean ZI of 10-22 mm and MIC of 0.78 – 22 µg/ml.

Aromatic medicinal plants bacteriauria E. coli resistance

References (43)

  1. 1 Antimicrobial activity of essential oils and other plant extracts [DOI]
  2. 2 Urinary Tract Infection [DOI]
  3. 3 Antimicrobial and phytochemical studies on 45 Indian medicinal plants against multi-drug resistant human pathogens [DOI]
  4. 4 Solid- and Vapor-Phase Antimicrobial Activities of Six Essential Oils: Susceptibility of Selected Foodborne Bacterial and Fungal Strains [DOI]
  5. 5 Increasing Antimicrobial Resistance and the Management of Uncomplicated Community-Acquired Urinary Tract Infections [DOI]
  6. 6 The etiology of urinary tract infection: traditional and emerging pathogens [DOI]
  7. 7 The etiology of urinary tract infection: Traditional and emerging pathogens [DOI]
  8. 8 Epidemiology of urinary tract infections [DOI]
  9. 9 Essential Oils of Aromatic Plants with Antibacterial, Antifungal, Antiviral, and Cytotoxic Properties – an Overview [DOI]
  10. 10 Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities [DOI]
  11. 11 Acute Uncomplicated Urinary Tract Infection in Women [DOI]
  12. 12 Antifungal activity of the lemongrass oil and citral against Candida spp. [DOI]
  13. 13 Bacterial Uropathogens in Urinary Tract Infection and Antibiotic Susceptibility Pattern in Jimma University Specialized Hospital, Southwest Ethiopia [DOI]
  14. 14 A Modern Look at Folkloric Use of Anti-infective Agents [DOI]
  15. 15 Urinary tract infection in adults: research priorities and strategies [DOI]
  16. 16 Antibacterial susceptibility of some urinary tract pathogens to commonly used antibiotics [DOI]
  17. 17 Study of bacteria isolated from urinary tract infections and determination of their susceptibility to antibiotics.
  18. 18 The synergistic preservative effects of the essential oils of sweet basil (Ocimum basilicum L.) against acid-tolerant food microflora [DOI]
  19. 19 Atividade antibacteriana de óleos essenciais em cepas isoladas de infecção urinária [DOI]
  20. 20 Im vitro antifungal activity of essential oils obtained from officinal plants against dermatophytes [DOI]
  21. 21 Urinary Infection in Pregnancy [DOI]
  22. 22 Antidiarrhoeal activities of Ocimum gratissimum (Lamiaceae).
  23. 23 Urinary tract infections in South Croatia: aetiology and antimicrobial resistance [DOI]
  24. 24 Prevalence and Antibiotics Susceptibility of Uropathogens in Patients from a Rural Environment, Tamilnadu [DOI]
  25. 25 Microbial aetiology and sensitivity of asymptomatic bacteriuria among ante-natal mothers in Mulago hospital, Uganda.
  26. 26 Catheter‐associated urinary tract infections: prevalence of uropathogens and pattern of antimicrobial resistance in a UK hospital (1996–2001) [DOI]
  27. 27 Urinary tract infections in women [DOI]
  28. 28 Antimicrobial activities of some ocimum species grown in rwanda [DOI]
  29. 29 The impact of resistance on the management of urinary tract infections [DOI]
  30. 30 The anti-mycobacterial activity of Lantana camara a plant traditionally used to treat symptoms of tuberculosis in South-western Uganda.
  31. 31 Resistance to Extended-Spectrum Cephalosporins and Mortality in Patients with Citrobacter freundii Bacteremia [DOI]
  32. 32 Potentiation of Antimicrobial Activity of Ciprofloxacin by Pelargonium graveolens Essential Oil against Selected Uropathogens [DOI]
  33. 33 Bacteriuria among adult non-pregnant women attending Mulago hospital assessment centre in Uganda.
  34. 34 Bioactive Botanicals from Basil ( Ocimum sp. )
  35. 35 Antimicrobial Effects of Essential Oils against Uropathogens with Varying Sensitivity to Antibiotics [DOI]
  36. 36 A survey on epidemiology of urinary tract infections and resistance pattern of uropathogens in an Iranian 1000-bed tertiary care hospital. [DOI]
  37. 37 Antibacterial and antifungal study of some essential oils and some of their constituents.
  38. 38 The antifungal activity of the leaf gland volatile oil of sweet gale (Myrica gale) (Myricaceae) [DOI]
  39. 39 Phytochemical and antimicrobial studies on essential oils of some aromatic plants.
  40. 40 [Antibacterial action of essential oils extracted from Madagascar plants].
  41. 41 Antiseptics in the Long‐term Urological Management of Patients by Intermittent Catheterization [DOI]
  42. 42 Antimycotic Activity of Essential Oils: The Possibility of Using New Bioactive Products Derived from Plants [DOI]
  43. 43 Principles and methods

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