Combination Assay for Tumor Markers in Saliva of Potentially Malignant Disorders and Oral Squamous Cell Carcinoma

Abstract

Potentially malignant disorders are “the risk of malignancy being present in a lesion or condition either at the time of initial diagnosis or at a future date” include mainly leukoplakia, erythroplakia, oral lichen planus, oral submucous fibrosis. Biomarkers were of immense help in diagnosis in recent years but a very few molecular biomarkers have been reported in the literature which are not significantly accurate. The current study aims at quantifying the levels of Transforming growth factor (TGF- β), Platelet derived growth factors (PDGF) and receptor of advanced glycosylation end products (RAGE) in saliva of patients with potentially malignant disorders and oral squamous cell carcinoma. Unstimulated saliva of oral squamous cell carcinoma and potentially malignant disorder patients was collected and stored in sub zero temperature. Further biochemical analysis was performed with Raybio ELISA kits. One way ANOVA was performed. The mean concentration of RAGE, PDGF and TGF- β was increased in oral squamous cell carcinoma groups and potentially malignant disorders when compared to healthy controls.p-0.000 (p<0.05).These combined assays can be used as potential biomarkers for indicating the prognosis of the disease and can be used as a diagnostic tool for screening and early detection as these combined assays give more reliable and accurate diagnosis when compared to single biomarker assays.