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Research Article Open access CC BY 4.0

Properties of Alpha-amylase of Lactobacillus plantarum Isolated from Cassava Waste Samples

Anthony Abiodun Onilude, Gbenga Solomon Ayinla, Chika Eluehike

Biotechnology Journal International · pp. 1–14 · Published 28 Jul 2017

10.9734/BJI/2017/33985

Abstract

The search for various industrial enzymes including starch degrading enzymes has received a great deal of attention for their perceived technological and economic benefits. Alpha-amylase has been produced from large varieties of organisms including pathogenic ones which are not Generally Regarded as Safe (GRAS). In this study, a total of Thirty nine (39) lactic acid bacteria were isolated from the different cassava waste samples (peels, cassava waste water, fibre and soil). The isolates were screened for their ability to produce α-amylase on starch agar. The amylolytic activity of the selected isolates was determined using starch-iodine complex while the enzymes were characterized based on parameters such as pH, temperature, substrate concentration, and metal ions. Upon starch hydrolysis, 29.9% of the LAB isolates demonstrated amylolytic properties with L. plantarum having the highest occurrence. The selected amylase producer was identified both phenotypically and molecularly as L. plantarum S7. Enzyme production was found to be highest (10.573U/ml) at 30 hours of incubation. Optimum temperature and pH for the enzyme activity was found to be 40°C and 7 respectively. The α-amylase retained more than 50% of it residual activity at pH 7 and 8 after preincubation for 90mins. Calcium chloride (CaCl₂) and Ammonium disulphate (NH₄ (SO₄)₂) enhanced amylase activity however, the  activity was inhibited at 5mM molar concentration by KCl, BaCl₂, CuSO₄, NaCl, EDTA (10%) and Urea (10%). The genomic level confirmation was done with 16s rDNA and the sequence showed 90% sequence similarity with L. plantarum.

Lactobacillus plantarum Cassava wastes amylase amylolytic lactic acid bacteria

References (28)

  1. 1 Molecular Cloning: A Laboratory Manual
  2. 2 Phylogenetic identification and in situ detection of individual microbial cells without cultivation [DOI]
  3. 3 Application of microbial α-amylase in industry - A review [DOI]
  4. 4 Amylases and their applications [DOI]
  5. 5 Production and biochemical characterization of an α-amylase from the moderate halophileHalomonas meridiana [DOI]
  6. 6 New efficient amylase-producing strains of Lactobacillus plantarum and L. fermentum isolated from different Nigerian traditional fermented foods [DOI]
  7. 7 Influence of pH and initial lactate concentration on the growth of Lactobacillus plantarum [DOI]
  8. 8 Isolation and characterization of new amylolytic strains of Lactobacillus fermentum from fermented maize doughs (mawè and ogi) from Benin [DOI]
  9. 9 Microbial and Physiological Characterization of Weakly Amylolytic but Fast-Growing Lactic Acid Bacteria: a Functional Role in Supporting Microbial Diversity in Pozol, a Mexican Fermented Maize Beverage [DOI]
  10. 10 The complete genomes of Lactobacillus plantarum and Lactobacillus johnsonii reveal extensive differences in chromosome organization and gene content [DOI]
  11. 11 Identification of Lactobacilli and Bifidobacteria [DOI]
  12. 12 Molecular characterization of nocardioform actinomycetes in activated sludge by 16S rRNA analysis [DOI]
  13. 13 Effect of fermentation by amylolytic lactic acid bacteria, in process combinations, on characteristics of rice/soybean slurries: A new method for preparing high energy density complementary foods for young children [DOI]
  14. 14 Starch‐modifying enzymes of lactic acid bacteria – structures, properties, and applications [DOI]
  15. 15 Partial characterization of α-amylase in the salivary glands of Lygus hesperus and L. lineolaris [DOI]
  16. 16 Comparison of amylolytic properties of Lactobacillus amylovorus and of Lactobacillus amylophilus [DOI]
  17. 17 Kinetics of Thermal Inactivation of the Extracellular Proteinase from Pseudomonas fluorescens 22F: Influence of pH, Calcium, and Protein [DOI]
  18. 18 Phenotypically based taxonomy using API 50CH of lactobacilli from Nigerian ogi, and the occurrence of starch fermenting strains [DOI]
  19. 19 Characterization of starch-hydrolyzing lactic acid bacteria isolated from a fermented fish and rice food, “burong isda”, and its amylolytic enzyme [DOI]
  20. 20 Assessing Microbial Community Diversity Using Amplicon Length Heterogeneity Polymerase Chain Reaction [DOI]
  21. 21 Detection of Active Butyrate-Degrading Microorganisms in Methanogenic Sludges by RNA-Based Stable Isotope Probing [DOI]
  22. 22 Partial Purification and Characterization of a Heat Stable α-Amylase from a Thermophilic Actinobacteria,Streptomycessp. MSC702 [DOI]
  23. 23 Production and characterization of amylase produced by Bacillus megaterium isolated from a local yam peel dumpsite in Minna, Niger State [DOI]
  24. 24 Fermented Cereal Products [DOI]
  25. 25 Enzyme Activities and Substrate Degradation by Fungal Isolates on Cassava Waste During Solid State Fermentation [DOI]
  26. 26 Amylolytic lactic acid bacteria in fish silage [DOI]
  27. 27 Amylase from Lactobacillus cellobiosus isolated from vegetable wastes
  28. 28 Biotechnological Potential of Alpha Amylase Production by Bacillus subtilis Using Cassava Peel Powder as a Substrate [DOI]

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