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Production of Bioethanol from Wild Cassava Manihot glaziovii through Various Combinations of Hydrolysis and Fermentation in Stirred Tank Bioreactors

Anselm P. Moshi, Ken. M. M. Hosea, Emrode Elisante, Anthony Manoni Mshandete, Ivo Achu Nges

Biotechnology Journal International · pp. 123–139 · Published 31 Dec 2014

10.9734/BBJ/2015/13981

Abstract

Production of Bioethanol from Wild Cassava Manihot glaziovii through Various Combinations of Hydrolysis and Fermentation in Stirred Tank Bioreactors Aim of the Study: The aim of this study was to evaluate three ethanol fermentation approaches namely (I) separate hydrolysis and common fermentation (II) separate hydrolysis and fermentation and (III) simultaneous saccharification and fermentation in stirred tank reactors using inedible wild cassava as feedstock. Study Design: Tubers of wild cassava (Manihot glaziovii) were obtained from two districts in Tanzania. Fermentation of hydrolysate and partially liquefied cassava flour was performed in stirred tank reactors.   Methodology: Feedstock composition analysis for structural carbohydrate was performed using acid hydrolysis and high pressure liquid chromatography technique. Analysis of total nitrogen was done by Kjeldahl acid digestion technique, total cyanide was determined using linamarase loaded picrate paper whereas macro-and micronutrients were analysed by inductively coupled plasma atomic emission spectrometry. Thermostable α-amylase and glucoamylase were used to partially hydrolyze the cassava flour to fermentable sugars prior to yeast fermentation. The hydrolysis (liquefaction) was performed at 90°C, 1h followed by saccharification using glucoamylase at 60°C, 2h for approaches I and II. For approach III, liquefaction was performed at 90°C, 1h followed by direct saccharification and fermentation. Fermentation of hydrolysate and partially liquefied starch from wild cassava was done in stirred tank reactors at 30±2°C using baker’s yeast. Place and Duration of Study: Department of Biotechnology, Lund University from January to June 2014. Results: The wild cassava (M. glaziovii) tubers possessed comparable physical dimensions to the domesticated cassava, however they displayed higher average flesh proportion (76 to 79%) compared to the domesticated cassava (74%). Compositional analysis disclosed that the wild cassava possessed interesting properties for bioethanol production such as dry matter of up to 89% w/w, degradable carbohydrate up to 90% (dry weight basis), total kjeldahal nitrogen 0.8-1.6% w/w and satisfactory concentration of macro-and micronutrients. Amongst the three fermentation approaches, high ethanol titre of 10-11% (v/v) at high conversion efficiency of 97.6% was achieved for separate hydrolysis and fermentation and simultaneous saccharification and fermentation, whereas low ethanol titre (4.2% v/v) at efficiency of 39% was achieved for separate hydrolysis and common fermentation. Volumetric productivities for the three approaches; ‘separate hydrolysis and common fermentation’, ‘separate hydrolysis and fermentation’, and ‘simultaneous saccharification and fermentation’ were 2.0, 5.5 and 6.5 respectively. Conclusion: The results obtained in the present study demonstrated that wild cassava has a high starch content, contain balanced nutrients required for efficient bioethanol production and that simultaneous saccharification and fermentation is the best approach for bioconversion of the wild cassava to bioethanol using stirred tank reactors.  

Bioethanol fermentation hydrolysis wild cassava

References (47)

  1. 1 Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar [DOI]
  2. 2 Features of promising technologies for pretreatment of lignocellulosic biomass [DOI]
  3. 3 Determination of Structural Carbohydrates and Lignin in Biomass
  4. 4 Trends in biotechnological production of fuel ethanol from different feedstocks [DOI]
  5. 5 Coordinated development of leading biomass pretreatment technologies [DOI]
  6. 6 Bioethanol production using carbohydrate-rich microalgae biomass as feedstock [DOI]
  7. 7 A Rapid Method for the Analysis of Starch [DOI]
  8. 8 Determination Of Total Nitrogen In Plant Tissue [DOI]
  9. 9 A comparison between simultaneous saccharification and fermentation and separate hydrolysis and fermentation using steam-pretreated corn stover [DOI]
  10. 10 Hemicelluloses negatively affect lignocellulose crystallinity for high biomass digestibility under NaOH and H2SO4 pretreatments in Miscanthus [DOI]
  11. 11 Comparison of Two Methods for Assaying Reducing Sugars in the Determination of Carbohydrase Activities [DOI]
  12. 12 Picrate paper kits for determination of total cyanogens in cassava roots and all forms of cyanogens in cassava products [DOI]
  13. 13 Comparison of SHF and SSF processes for the bioconversion of steam-exploded wheat straw [DOI]
  14. 14 Production of ethanol from cassava pulp via fermentation with a surface-engineered yeast strain displaying glucoamylase [DOI]
  15. 15 Improved bio-energy yields via sequential ethanol fermentation and biogas digestion of steam exploded oat straw [DOI]
  16. 16 Challenges in enzymatic hydrolysis and fermentation of pretreated Arundo donax revealed by a comparison between SHF and SSF [DOI]
  17. 17 Zinc and yeast stress tolerance: Micronutrient plays a big role [DOI]
  18. 18 Anaerobic aniline degradation via reductive deamination of 4-aminobenzoyl-CoA in Desulfobacterium anilini [DOI]
  19. 19 Energy efficiency and potentials of cassava fuel ethanol in Guangxi region of China [DOI]
  20. 20 Biological cyanide destruction mediated by microorganisms [DOI]
  21. 21 The effects of pantothenate deficiency and acetate addition on anaerobic batch fermentation of glucose by Saccharomyces cerevisiae [DOI]
  22. 22 Variation in Properties of Starch in Cassava Varieties in Relation to Age of the Crop [DOI]
  23. 23 Bio-Ethanol Production from Non-Food Parts of Cassava (Manihot esculenta Crantz) [DOI]
  24. 24 Production of raw cassava starch-degrading enzyme by Penicillium and its use in conversion of raw cassava flour to ethanol [DOI]
  25. 25 Importance of policy support and feedstock prices on economic feasibility of bioethanol production from wheat straw in the UK [DOI]
  26. 26 Life cycle cost analysis of fuel ethanol produced from cassava in Thailand [DOI]
  27. 27 Characterization of a New Obligately Anaerobic Thermophile, Thermoanaerobacter wiegelii sp. nov. [DOI]
  28. 28 A consolidated bio-processing of ethanol from cassava pulp accompanied by hydrogen production [DOI]
  29. 29 High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System [DOI]
  30. 30 Combined product and substrate inhibition equation for cellobiase [DOI]
  31. 31 Characterisation of some properties of cassava root tubers [DOI]
  32. 32 High Level Ethanol from Sugar Cane Molasses by a New ThermotolerantSaccharomyces cerevisiaeStrain in Industrial Scale [DOI]
  33. 33 Studies and Preliminary Design for a Cassava Tuber Peeling Machine [DOI]
  34. 34 Theory of a mechanical method of peeling cassava tubers with knives
  35. 35 EFFECT OF ZINC AND COBALT ON YEAST GROWTH AND FERMENTATION [DOI]
  36. 36 Biofuels in Tanzania: Status, Opportunities and Challenges
  37. 37 Use of Response Surfaces to Investigate Metal Ion Interactions in Yeast Fermentations [DOI]
  38. 38 Saccharification of cassava flour starch in a hollow-fiber membrane reactor [DOI]
  39. 39 Wild cassava, Manihot spp.: Biology and potentialities for genetic improvement [DOI]
  40. 40 Effect of pH, aeration and feeding non‐sterilized agave juice in a continuous agave juice fermentation [DOI]
  41. 41 Assessment of waxy and non-waxy corn and wheat cultivars as starch substrates for ethanol fermentation [DOI]
  42. 42 Effect of physical and mechanical properties of cassava tubers on the performance of an automated peeling machine
  43. 43 Acetate synthesis from 2 CO2 in acetogenic bacteria: is carbon monoxide an intermediate? [DOI]
  44. 44 Membrane reactors for the investigation of product inhibition of enzyme activity [DOI]
  45. 45 Effect of some vitamins and micronutrient deficiencies on the production of higher alcohols by Saccharomyces cerevisiae [DOI]
  46. 46 An Evaluation of Different Bioreactor Configurations with Immobilized Yeast for Bioethanol Production [DOI]
  47. 47 Single-Step Bioconversion of Unhydrolyzed Cassava Starch in the Production of Bioethanol and Its Value-Added Products [DOI]

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