Potential Pathogenicity of Escherichia coli Isolated from the Stools of Healthy Children Suffering from Diarrhea Admitted to Hospitals in  Southern Benin

Durand Dah-Nouvlessounon; Haziz Sina; Annatou Yakoubou; Bawa Boya; Scylax Azatassou; Christine N’Tcha; Agossou Damien Pacôme Noumavo; Funkè F. Assouma; Adolphe Adjanohoun; Lamine Baba-Moussa

doi:10.9734/jamb/2023/v23i12773

Research Article Open access CC BY 4.0

Potential Pathogenicity of Escherichia coli Isolated from the Stools of Healthy Children Suffering from Diarrhea Admitted to Hospitals in Southern Benin

Durand Dah-Nouvlessounon, Haziz Sina, Annatou Yakoubou, Bawa Boya, Scylax Azatassou, Christine N’Tcha, Agossou Damien Pacôme Noumavo, Funkè F. Assouma, Adolphe Adjanohoun, Lamine Baba-Moussa

Journal of Advances in Microbiology · pp. 15–29 · Published 14 Dec 2023

10.9734/jamb/2023/v23i12773

Abstract

Aims: Infant mortality linked to infectious diseases remains a major public health concern, especially in the context of antibiotic resistance. This work aimed to study the potential pathogenicity of Escherichia coli strains isolated from the stools of children suffering from diarrhea and healthy from 0 to 15 years old admitted to hospitals in southern Benin. Methodology: The isolation and characterization of the strains were carried out according to standard microbiology methods. Molecular characterization was carried out by searching for eight virulence factors (Stx1, Stx2, eaeA, bfpB, LT, InVE, IpaH, AggR) and three antibiotic resistance genes (blaTEM, blaSHV, blaCTX -M) by the PCR technique. Results: This study shows that 47.06% of the children sampled suffered from diarrhea, against 52.94% who were healthy. A total of 9 species of Enterobacteriaceae were identified in different proportions: Escherichia coli (66.66%), Klebsiella ornithinolytica (13.72%), Klebsiella pneumonia (5.88%), Enterobacter aerogenes (3.92%), Escherichia vulneris (1.96%), Salmonella arizonae (1.96%), Salmonella spp (1.96%), Enterobacter sakazakii (1.96%), Serratia fonticola (1.96%). All E. coli showed total resistance to amoxicillin, ceftazidime, and cefotaxime. In addition, they showed variable resistance to norfloxacin (95.33%), trimethoprim (83.33%), and sulfamethoxazole (95.24%). 70.83% of E. coli strains isolated from apparently healthy children carry resistance genes blaTEM (12.5%), blaSHV (25%), and blaCTX-M (33.33%). While 66.67% of strains of E. coli isolated from children with diarrhea carry resistance genes blaTEM (12.5%), blaSHV (37.5%), and blaCTX-M (16.67%). Only EPEC was isolated and only in diarrheal children, with a frequency of 20.83%. Conclusion: The relatively high prevalence of ECD in this study is remarkable, and it highlights the fact that E. coli is an important agent of infectious diarrhea and could be the leading cause of gastroenteritis in Benin.

Pathogenic E. coli antimicrobial resistance molecular characterization southern Benin

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Durand Dah-Nouvlessounon, Haziz Sina, Annatou Yakoubou, Bawa Boya, Scylax Azatassou, Christine N’Tcha, Agossou Damien Pacôme Noumavo, Funkè F. Assouma, Adolphe Adjanohoun, Lamine Baba-Moussa (2023). Potential Pathogenicity of Escherichia coli Isolated from the Stools of Healthy Children Suffering from Diarrhea Admitted to Hospitals in Southern Benin. Journal of Advances in Microbiology , 15–29. https://doi.org/10.9734/jamb/2023/v23i12773

Affiliations

Durand Dah-Nouvlessounon — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Haziz Sina — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Annatou Yakoubou — Pediatric Unit, Hospital and University Center for Mothers and Children Lagune (CHU-MEL) of Cotonou, Benin.
Bawa Boya — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Scylax Azatassou — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Christine N’Tcha — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Agossou Damien Pacôme Noumavo — Laboratory of Microbiology and Food Technology, Department of Plant Biology, Cotonou, University of Abomey-Calavi, Benin.
Funkè F. Assouma — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.
Adolphe Adjanohoun — National Agronomic Research Institute of Benin, 01 BP 884 Cotonou, Republic of Benin.
Lamine Baba-Moussa — Laboratory of Biology and Molecular Typing in Microbiology, Department of Biochemistry and Cell Biology, University of Abomey-Calavi, Benin.

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