Phytochemical Screening and Quantitative Estimation of Quercetin and Gallic Acid in Ethanolic Extract of Moringa oleifera Leaves by HPTLC
Jyoti, R.K. Sharma, Pankaj Kumar Umar, Kshemankar Shrman, Sachin Jain, VIDHI GAUTAM, Anil Gattani, Shashank Vishvakarma
Journal of Advances in Biology & Biotechnology · pp. 1193–1202 · Published 19 Nov 2025
10.9734/jabb/2025/v28i113311Abstract
Moringa oleifera (family Moringaceae), commonly known as the “Miracle Tree,” is widely recognized for its rich nutritional and therapeutic properties. The present study investigated the phytochemical composition and quantified major antioxidant compounds, quercetin and gallic acid, in ethanolic leaf extract of M. oleifera using High-Performance Thin Layer Chromatography (HPTLC). Preliminary phytochemical screening confirmed the presence of carbohydrates, phenols, tannins, triterpenoids, flavonoids, and alkaloids. Quantitative estimation by HPTLC revealed quercetin and gallic acid contents of 860 µg/g (0.08%) and 273 µg/g (0.03%), respectively. The results validate the antioxidant richness of M. oleifera and support its potential application in pharmaceutical and nutraceutical formulations.
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