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Research Article Open access CC BY 4.0

Phytochemical Screening, In vitro Antioxidant Capacity and Nephro-protective Effects of Combined Extract of Psidium guajava and Carica papaya Leaves in Rats Intoxicated with Cadmium

O. C. Orororo, O. Efekemo, O. B. Odeghe, P. D. Clark, E. P. Awhin, E. O. Egbune, I. O. Efejene

Asian Journal of Medicine and Health · pp. 86–97 · Published 25 Jul 2024

10.9734/ajmah/2024/v22i81073

Abstract

Aim: Toxic effects arising from heavy metals and other contaminants in the environment are presently been tackled by the use of medicinal plants. Thus, this study evaluated the protective effects of combined extract of Psidium guajava and Carica papaya leaves on kidney injury occasioned by cadmium (Cd) toxicity. Materials and Methods: Phytochemical investigation of the combined extract revealed the presence of tannins, saponins, flavonoids, triterpene, steroids, alkaloids, cardiac glycoside, anthocyanins, and anthraquinone and the the extract displayed excellent antioxidant capacity in vitro. Eighteen adult female rats were divided into three groups: Group 1 (Control, received feed and water only); Group 2 (Cadmium, received single dose of cadmium, 30mg/kg body weight at the start of the experiment) and Group 3 (Cadmium + combined leaf extract, received cadmium as in group 2 above and 200mg/kg body weight of extract, daily for two weeks). Results: Intoxication with Cd causes significantly (p<0.05) increased in the levels of lipid peroxidation and in the activity of glutathione peroxidase. Cd intoxication also depleted glutathione (GSH), super oxide dismutase (SOD) and catalase (CAT) reserves and brought about a significant increase in serum urea, creatinine, sodium and potassium. However a significant (p<0.05) normalization in the levels of GSH, SOD, CAT, urea, creatinine, sodium and potassium was observed in Cd-intoxicated rats administered the extracts as compared to the control (untreated rats). Conclusion: The results reveal that Cd causes nephro-toxicities manifested via lipid peroxidation, increase in serum levels of urea, creatinine, sodium and potassium ions and that the combined extract of Psidium guajava and Carica papaya leaves possess adequate antioxidant power against Cd-induced nephro-toxic effects.

Psidium guajava Carica papaya phytochemicals antioxidant capacity

References (31)

  1. 1 Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.
  2. 2 The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase [DOI]
  3. 3 Improved method for the determination of blood glutathione.
  4. 4 Colorimetric assay of catalase [DOI]
  5. 5 Antioxidant responses and cellular adjustments to oxidative stress [DOI]
  6. 6 Phytochemical Screening and Antioxidant Activities of Some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria [DOI]
  7. 7 Effects of Calmodulin Antagonists on Radiation-induced Lipid Peroxidation in Microsomes [DOI]
  8. 8 Cellular mechanisms of cadmium-induced toxicity: a review [DOI]
  9. 9 The phytochemistry and medicinal value of Psidium guajava (guava) [DOI]
  10. 10 Cadmium-Induced Pathologies: Where Is the Oxidative Balance Lost (or Not)? [DOI]
  11. 11 Toxicity assessment due to sub-chronic exposure to individual and mixtures of four toxic heavy metals [DOI]
  12. 12 Toxicological assessment of combined lead and cadmium: Acute and sub-chronic toxicity study in rats [DOI]
  13. 13 The Role of Oxidative Stress in Carcinogenesis Induced by Metals and Xenobiotics [DOI]
  14. 14 Antioxidant activities of Moringa oleifera leaf extract against pendimethalin-induced oxidative stress and genotoxicity in Nile tilapia, Oreochromis niloticus (L.) [DOI]
  15. 15 Evaluation of Antioxidant and Free Radical Scavenging Capacities of Some Nigerian Indigenous Medicinal Plants [DOI]
  16. 16 The role of intracellular oxidation in death induction (apoptosis and necrosis) in human promonocytic cells treated with stress inducers (cadmium, heat, X-rays) [DOI]
  17. 17 Protective effect of the aqueous leaf and seed extract of Phyllanthus amarus on gentamicin and acetaminophen-induced nephrotoxic rats [DOI]
  18. 18 Selective anti-proliferative activities of Carica papaya leaf juice extracts against prostate cancer [DOI]
  19. 19 Phytochemical Screening and Mineral Composition of the Leaves of Ocimum gratissimum (Scent Leaf) [DOI]
  20. 20 Protective effect of Vitamin C and E on enzymatic and antioxidant system in liver and kidney toxicity of Cadmium in rats [DOI]
  21. 21 Protective role of N-Acetyl L-Cysteine against reproductive toxicity due to interaction of lead and cadmium in male Wistar rats [DOI]
  22. 22 A comparison with antioxidant and functional properties among five mango (Mangifera indica L.) varieties in Bangladesh.
  23. 23 Phytochemical screening and bioactivity assessment of leaves and fruits extract of Carica papaya [DOI]
  24. 24 The Role of Urinary Cadmium and Lead Level on Pregnant Women Renal Function [DOI]
  25. 25 Anti-Lipid Peroxidation Activities of Three Selected Fruits Juices Against Cadmium Induced Liver Damage In Vitro [DOI]
  26. 26 Phytochemical screening and antioxidant activity of Psidium guajava
  27. 27 Role of Anthocleista vogelii in serum antioxidant defence system in cadmium-induced oxidative stress in Wistar rats [DOI]
  28. 28 The Role Formation of Methylglyoxal, Carbonyl Compound, Hydrogen Peroxide and Advance Oxidation Protein Product Induced Cadmium in Ovarian Rat [DOI]
  29. 29 Comparative phytochemical screening, in vivo antioxidant and nephroprotective effects of extracts of cassava leaves on paracetamol-intoxicated rats [DOI]
  30. 30 In vitro antioxidant, anti-lipid peroxidation activities and HPLC analysis of methanol extracts from bark and stem of Mahonia leschenaultia takeda
  31. 31 Beta Carotene as an Antioxidant in Cadmium Induced Testicular Toxicity

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