Pharmacological and Phytochemical Evaluation of Ethonalic Extract of Ixora brachiata (Roxb) Leaves

Abstract

Objective: Current scientific investigations have evidenced that there is increased attention in developing novel drugs from bio source for the human health concern. This study's main objective was to intervene phytochemical constituents present in the ethanol leaf extract of an aromatic plant, Ixora brachiata (Roxb) and to evaluate their ability on antioxidant, anticancer and larvicidal properties, using invitro models. Methods: Antioxidant assay was performed by scavenging behaviour of DPPH, ABTS, H2 O2 and Reducing power screenings. The phytochemical composition was also screened using GC-MS technique. Cytotoxicity effect was determined by employing MTT Assay, the larvicidal potential was predicted by following the protocol described by WHO. In phytochemical screening higher concentration of alkaloids, flavonoids, phenolic and diterpenoids were detected in significant content. Geranyl linalool was the profound compound present, and it has been reported, used in various treatments for cancer ailments, similar to Taxol. Results: The cytotoxicity values of ethanol extract were found to be 11.57 to 55.95% against the cancer cells (A549) and 12.36-63.8% range was observed against HeLa cancers cell line. Larvicidal effects of the extract showed minimum LC50 value 14.69mg/ml against Aedes aegypti  and 10.43mg/ml was recorded, against Culex quinquefasciatus, indicating, a promising capability of larvicidal action against mosquito larvae. Conclusions: This study envisaged that ethanol extracts of I.brachiata are a bright and capable therapeutic agent, to combat in the battle of life threatened diseases, and infectious emerging threats.