Pectinase Production from Saccharomyces Cerevisiae Using Orange Peels and Maize Cobs as Substrate for Solid-State Fermentation
M. S. Haske, M. Y. Iliyasu, A. Abdulrahman, S. M. Sani, T. Inusa, S. Isma’il, R. D. Umar, Z. M. Kabeer, H. Tahir, E. B. Agbo
Journal of Applied Life Sciences International · pp. 49–56 · Published 10 Apr 2023
10.9734/jalsi/2023/v26i2601Abstract
Background of the Study: Degradation of wastes especially fruit waste is important because these wastes accumulate in the environment. The fruit wastes can be used to biosynthesize pectinase enzyme at a cheaper rate due its numerous industrial applications. The ability of Saccharomyces cerevisiae to degrade these wastes to produce pectinase enzyme was exploited. Aim: The aim of this study is to isolate and characterize Saccharomyces cerevisiae and study the optimal conditions needed for pectinase production on the orange peel with maize cobs and study the effectiveness of the enzymes produced in the extraction of orange juice. Place and Duration of Study: The study was conducted at the Microbiology Laboratory of Abubakar Tafawa Balewa University Bauchi, Bauchi State, Nigeria, between August to September 2021. Methodology: Soil samples from the orange seller stand was collected, serial dilution method was used to reduce the solution to a more usable concentration, and the samples were streaked on Yeast Peptone Dextrose Agar (YPDA). The plates were incubated for 3 to 9 days at 28+- oC. The isolated yeast was identified based on the morphological and biochemical test, the isolates were inoculated on the prepared fermentation media under different optimal conditions. The crude enzyme were extracted, and the extracts were tested for pectinase activity using the dinitrosalicylic reagent (DNS) method for the presence of reducing sugar. The resulting pectinase was determined to effectively extract juice from orange mash. Results: Saccharomyces cerevisiae showed relatively pectinase hydrolysis using orange peels and maize cobs as substrate by solid-state fermentation under predetermined optimum fermentation conditions. Optimization for the pectinase production was done by altering the conditions for the production medium. Pectinase activity of (2.81 + 2.31 ug/ml) was observed at 72hr of incubation at 35oC. Conclusion: The study demonstrates that orange peel and maize cob can be successfully used to induce the production of pectinase using Saccharomyces cerevisiae under solid-state fermentation.
References (17)
- 1 Methods for Isolation, Phenotypic Characterization and Maintenance of Yeasts [DOI]
- 2 The Use of Solid Media for Detection of Enzyme Production by Fungi [DOI]
- 3 Cellulose, hemicelluloses, lignin and ash content of some organic materials and their suitability for use as paper pulp supplements [DOI]
- 4 The Use of Solid Media for Detection of Enzyme Production by Fungi [DOI]
- 5 Production, Characterization, and Industrial Application of Pectinase Enzyme Isolated from Fungal Strains [DOI]
- 6 Use of Saccharomyces cerevisiae cells immobilized on orange peel as biocatalyst for alcoholic fermentation [DOI]
- 7 Biochemical Prospects of Various Microbial Pectinase and Pectin: An Approachable Concept in Pharmaceutical Bioprocessing [DOI]
- 8 Citrus science and technology
- 9 Pectinase: Substrate, Production and their Biotechnological Applications [DOI]
- 10 Fibre Fraction from Orange Peel Residues after Pectin Extraction [DOI]
- 11 Agro-residues as Alternative for Xylanase Production by Filamentous Fungi [DOI]
- 12 Papaya peel valorization for production of acidic pectin methylesterase by Aspergillus tubingensis and its application for fruit juice clarification [DOI]
- 13 Studies on the degradation of wood sawdust by Lentinus squarrosulus (Mont.) Singer [DOI]
- 14 Biochemical Engineering Aspects of Solid-State Fermentation [DOI]
- 15 Screening of Fungi from Disposed Maize Cobs for Amylase Production [DOI]
- 16 Sustainable Production of Bioethanol by Zymomonas mobilis and Saccharomyces cerevisiae using Rice Husk and Groundnut Shell as Substrates [DOI]
- 17 Phenotypic Detection of Cellulose Hydrolysing Bacteria from the Rumen of Cow and Goat in Bauchi Metropolis [DOI]
Cited by 1
Andréa dos Santos Oliveira, Fernanda Moreira Lemes · Editora Científica Digital eBooks · 2025
Related research
- Microbial Synthesis of Polyhydric Alcohol by Saccharomyces cerevisiae — shares topic coverage
- Influence of Saccharomyces cerevisiae (Baker’s Yeast) on the Fermentation of Ogi-A Nigerian Fermented Food — shares topic coverage
- An Electron Microscopic Study of the Effect of (Saccharomyces cerevisiae) on the Ability of E. coli 0157:H7 to Attach and Efface Healthy Young Broilers — shares topic coverage
- Comparative Studies on Production of Bioethanol from Rice Straw Using Bacillus subtilis and Trichoderma viride as Hydrolyzing Agents — shares topic coverage
- Evaluation of C19 Steroid Intermediates during Microbial Transformation of Phytosterol — shares topic coverage
Article metrics
Real usage data collected on this platform.
58
Page views
0
PDF downloads
7
Outbound clicks
1
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.