Integrating In-silico and In-vitro Approaches to Screen the Antidiabetic Drug from Trigonella foenum graecum Linn.

Abstract

One of cardiovascular diseases, i.e. Diabetes is reported either through absence of insulin or resistance against insulin. Treatment for diabetic disease related to Type 1 and 2 which includes lots of expenditure every year in the form of capsules or preparing different types of formulation. Recently, more than 400 medicinal plants have been screened to fulfill the requirement of the potent antidiabetic drug. Isolation and purification of effective herbal medicine from these medicinal plants to cure diabetes disease are still challenging. In the present study, our group worked on different enzymes that played a crucial role in the carbohydrate metabolism. For these studies, number of secondary metabolites (19) and enzymes (5) were screened in silico for antidiabetic potential. The result of these studies which showed that alpha amylase formed best interaction with carpaine (an alkaloid) with the lesser binding energy -7.44 kcal/mol as compared with the standard drug Acarbose. Therefore, further in-vitro studies were included, the screening of alpha amylase inhibitory activity using different solvent extract. At the concentration of 1 mg/ml FGCE showed highest inhibitory activity i.e. 37.7%. Thin layer chromatography (TLC) was also performed to confirm the number of phytochemicals present in different extract. Overall, these studies claimed that medicinal plant posses a potential antidiabetic activity, but still investigation is required to find out and purify the bioactive compound which can be served as a potent antidiabetic drug.