Skip to content
Research Article Open access CC BY 4.0

Morphological and SSR Assessment of Putative Drought Tolerant M3 and F3 Families of Wheat (Triticum aestivum L.)

A. M. M. Al-Naggar, Kh. F. Al-Azab, S. E. S. Sobieh, M. M. M. Atta

Biotechnology Journal International · pp. 174–190 · Published 18 Mar 2015

10.9734/BBJ/2015/15898

Abstract

In an attempt to develop drought tolerant genotypes of bread wheat, two procedures, i.e., mutation and hybridization were used to induce new genetic variation. Selection for high grain yield/plant (GYPP) and other desirable traits was practiced in the M2 populations of 7 gamma irradiated genotypes and F2 populations of 15 diallel crosses among 6 genotypes of wheat under well watering (WW) and water stress (WS) conditions. Progenies of these selections (53 M3 and 109 F3 families) and their seven parents were evaluated in the field under WW and WS. Significant yield superiority of twelve families (7 M3’s and 5 F2’s) over their original and better parents, respectively under WS reached 74.71% (SF9). These putative drought tolerant families were assessed on the DNA level using SSR analysis. Fifteen SSR primers were used for PCR amplification of the genomic DNA of these 12 selections and their parents. The SSR analysis proved that the 12 families are genetically different from their 7 parents, with an average polymorphism of 86.67%. The genetic similarities (Gs) ranged from 30% to 88%. Both mutants SF3 and SF4 exhibited very low Gs (42 and 40%, respectively) with their common parent (Giza-168), indicating that gamma rays were very effective in changing the genetic background of Giza-168 towards high GYPP under WS conditions. SSR assay permitted the identification of seven unique bands (5 positive and 2 negative) for three drought tolerant wheat genotypes (SF3, SF4 and Aseel-5). These bands might be considered useful as markers associated with drought tolerance in bread wheat breeding programs.

Putative mutants transgressive segregants bread wheat drought tolerance SSR markers genetic similarity

References (19)

  1. 1 Deviations from michaelis-menten behaviour of plant glutamate dehydrogenase with ammonium as variable substrate [DOI]
  2. 2 A Microsatellite Map of Wheat [DOI]
  3. 3 Survey of plant short tandem DNA repeats [DOI]
  4. 4 Evaluation of Methods for Quantification of Drought Tolerance in Wheat [DOI]
  5. 5 Genetic diversity and its relationship to hybrid performance and heterosis in rice as revealed by PCR-based markers [DOI]
  6. 6 Integration of Simple Sequence Repeat DNA Markers into a Soybean Linkage Map [DOI]
  7. 7 Analysis of simple sequence repeats (SSRs) in wild barley from the Fertile Crescent: associations with ecology, geography and flowering time [DOI]
  8. 8 DNA analyses and their applications in plant breeding [DOI]
  9. 9 Isolation of DNA for RAPD analysis from dry leaf material of someHesperis L. specimens [DOI]
  10. 10 Morphological and molecular genetic variation in wheat for salinity tolerance at germination and early seedling stage
  11. 11 Comparative analyses of the genetic diversity among bread wheat genotypes based on RAPD and SSR markers
  12. 12 EFFECT OF WATER STRESS ON YIELD AND YIELD COMPONENTS OF WHEAT (TRITICUM AESTIVUM L.) VARIETIES
  13. 13 SSR markers associated with membrane stability in wheat (Triticum aestivum L.).
  14. 14 Ecogeographic adaptation and genetic variation in wild barley, Application of molecular markers targeted to environmentally regulated genes [DOI]
  15. 15 Predicted and Actual Gain from Selection for Early Maturing and High Yielding Wheat Genotypes Under Water Stress Conditions [DOI]
  16. 16 Screening for Drought Tolerance Using Molecular Markers and Phenotypic Diversity in Durum Wheat Genotypes
  17. 17 MOLECULAR MARKERS FOR DROUGHT TOLERANCE IN BREAD WHEAT [DOI]
  18. 18 Genetic variation for salt tolerance in some bread and pasta wheat genotypes
  19. 19 Gene Effects Controlling Inheritance of Earliness and Yield Traits of Bread Wheat Under Drought Stress Conditions [DOI]

Cited by 11

Genetic parameters controlling the inheritance of glaucousness and yield traits in bread wheat

A. M. M. Al-Naggar, K. F. Al-Azab, A. S. M. Younis · Brazilian Journal of Biology · 2022

Performance and Genetic Diversity in Water Stress Tolerance and Relation to Wheat Productivity Under Rural Regions

Hassan Auda Awaad · Springer Water · 2021

Introduction to “Mitigating Environmental Stresses for Agricultural Sustainability in Egypt”

Hassan Auda Awaad, Abdelazim M. Negm, Mohamed Abu-hashim · Springer Water · 2021

Showing 3 of 11 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

15

Page views

0

PDF downloads

2

Outbound clicks

11

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.