Skip to content
Research Article Open access CC BY 3.0

Molecular Identification of Cassava Mosaic Begomoviruses Associated with Cassava Mosaic Disease in the DR-Congo Using Primer Pairs

Marcel Muengula-Manyi, Claude Bragard, Stephan Winter, Adrien Kalonji-Mbuyi, Kabwe K. C. Nkongolo, Djim Tshilenge-Kanana

Biotechnology Journal International · pp. 579–588 · Published 13 May 2014

10.9734/BBJ/2014/8112

Abstract

Molecular analysis of cassava leaf samples from different regions of the Democratic Republic of Congo (DR-Congo) was conducted to identify cassava mosaic begomoviruses (CMBs)  associated with cassava infections in farmers' fields in Gandajika (Eastern Kasai), Bas-Congo and Kinshasa. Four specific primer pairs M1F/M1R, M2F/M2R, Begomo 146/Begomo 672, and EAC4F/EAC4R were used to detect East African cassava mosaic virus (EACMV) AC2, African Cassava Mosaic Virus (ACMV) AC2, ACMV DNA-A, and EACMV-UG DNA-A AC4, respectively. Based on PCR analysis, only African cassava mosaic virus (ACMV) and East African cassava mosaic virus (EACMV-UG) were identified in the cassava growing regions. Overall, 67% of cassava samples were infected with ACMV, 10% with EACMV-UG, and 10% with both, ACMV and EACMV-UG. No virus was detected in 13% of symptomatic samples. Thus, although EACMV-UGG is present in targeted areas, ACMV remained the most common CMB in the DR-Congo. 

Polymerase chain reaction cassava mosaic disease African cassava mosaic virus east African cassava mosaic virus DR Congo

References (27)

  1. 1 Evidence that DNA-A of a geminivirus associated with severe cassava mosaic disease in Uganda has arisen by interspecific recombination. [DOI]
  2. 2 Evidence of synergism between African cassava mosaic virus and a new double-recombinant geminivirus infecting cassava in Cameroon [DOI]
  3. 3 Cassava Mosaic Virus Disease in East and Central Africa: Epidemiology and Management of A Regional Pandemic [DOI]
  4. 4 Methods of surveying the incidence and severity of cassava mosaic disease and whitefly vector populations on cassava in Africa: a review [DOI]
  5. 5 Host‐Plant Viral Infection Effects on Arthropod‐Vector Population Growth, Development and Behaviour: Management and Epidemiological Implications [DOI]
  6. 6 Molecular biodiversity of cassava begomoviruses in Tanzania: evolution of cassava geminiviruses in Africa and evidence for East Africa being a center of diversity of cassava geminiviruses [DOI]
  7. 7 African cassava mosaic virus disease: the magnitude of the problem.
  8. 8 Effects of African cassava mosaic geminivirus on the yield of cassava.
  9. 9 Dual begomovirus infections and high Bemisia tabaci populations: two factors driving the spread of a cassava mosaic disease pandemic [DOI]
  10. 10 Changes in the incidence of African cassava mosaic virus disease and the abundance of its whitefly vector along south–north transects in Uganda [DOI]
  11. 11 African Cassava Whitefly, Bemisia tabaci, Resistance in African and South American Cassava Genotypes [DOI]
  12. 12 Properties, relationships and distribution of cassava mosaic geminiviruses
  13. 13 Types of variation in DNA-A among isolates of East African cassava mosaic virus from Kenya, Malawi and Tanzania. [DOI]
  14. 14 The current pandemic of cassava mosaic virus disease in East Africa and its control.
  15. 15 Presence of a new virus closely related to East African cassava mosaic geminivirus, associated with cassava mosaic outbreak in Uganda.
  16. 16 The Viruses and Virus Diseases of Cassava in Africa
  17. 17 Occurrence of the Uganda variant of East African cassava mosaic virus (EACMV‐Ug) in western Democratic Republic of Congo and the Congo Republic defines the westernmost extent of the CMD pandemic in East/Central Africa [DOI]
  18. 18 East African cassava mosaic Zanzibar virus – a recombinant begomovirus species with a mild phenotype [DOI]
  19. 19 Dual infection by cassava begomoviruses in two leguminous species (Fabaceae) in Yangambi, Northeastern Democratic Republic of Congo [DOI]
  20. 20 Population dynamics ofBemisia tabaci(Homoptera: Aleyrodidae) parasitoids on cassava mosaic disease–resistant and susceptible varieties [DOI]
  21. 21 Farm Level Practices Relevant to Cassava Plant Protection
  22. 22 Viruses Associated with Cassava Mosaic Disease in Senegal and Guinea Conakry [DOI]
  23. 23 Incidence, Severity and Gravity of Cassava Mosaic Disease in Savannah Agro-Ecological Region of DR-Congo: Analysis of Agro-Environmental Factors [DOI]
  24. 24 Incidence of Cassava Viral Diseases and First Identification of East African cassava mosaic virus and Indian cassava mosaic virus by PCR in Cassava (Manihot esculenta Crantz) Fields in Togo [DOI]
  25. 25 Occurrence and Distribution of Cassava Mosaic Begomovirus Related to Agro-ecosystems in the Sud-kivu Province, Democratic Republic of Congo [DOI]
  26. 26 Molecular Evidence for the Association of a Strain of Uganda Variant of East African Cassava Mosaic Virus to Symptom Severity in Cassava (Manihot esculenta Crantz) Fields in Togo [DOI]
  27. 27 Variations and taxonomic status of begomoviruses causing severe epidemics of cassava mosaic disease in Kenya, Uganda, and Democratic Republic of the Congo [DOI]

Cited by 1

Epidemiology of Cassava Mosaic Disease Viruses in Western Democratic Republic of Congo

Mayanza Kingolo, A. Mumbumbu, Bakelana Zeyimo · Journal of Agricultural Sciences · 2026

Article metrics

Real usage data collected on this platform.

10

Page views

0

PDF downloads

1

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.