Molecular Characterization and Optimization of Alkaline Protease Production by Bacillus cereus LS23B
Adefunke Oluwaseun Ogunniran, Adeniyi Success Ohunayo, Iyanuoluwa Oluwaseun Daramola, Titilayo Olufunke Femi-Ola
Asian Journal of Biotechnology and Bioresource Technology · pp. 1–13 · Published 10 Nov 2023
10.9734/ajb2t/2023/v9i4188Abstract
The study aimed to determine the conditions leading to maximum protease production using submerged fermentation and detecting the presence of protease genes in the bacteria. This is necessary to meet the increasing demand for protease enzymes in the industrial market. The ability of the bacterial isolate to produce protease enzymes was evaluated through primary screening. After that, morphological characterization, biochemical tests, and 16S rRNA analysis were done to identify the bacterial strain. To confirm the presence of the gene-encoding enzyme, the protease (npr) gene primer was amplified using a polymerase chain reaction. The pH, incubation duration, temperature, carbon, and nitrogen sources were studied. Others include metal ions, substrate concentration, and agitation speed. The bacterial strain has 100% similarity to Bacillus cereus A9, while the protease encoding gene was confirmed with positive bands of 951bp. The enzyme was optimally produced at 40 oC with a pH of 9 after 72 h incubation. Starch, gelatin, 1 % substrate concentration, 1mM K+, and agitation speed of 160 rpm fully supported protease production. The presence of the npr gene in the isolate was confirmed. Also, the optimization study reveals that Bacillus cereus LS23B can be used in large-scale protease production, which may be used in different biotechnological applications.
References (31)
- 1 A review on the application of Bacillus as probiotics in aquaculture [DOI]
- 2 Biotechnological Applications of Proteases in Food Technology [DOI]
- 3 Proteases: History, discovery, and roles in health and disease [DOI]
- 4 Microbial proteases: ubiquitous enzymes with innumerable uses [DOI]
- 5 A Review on Microbial Alkaline Proteases
- 6 Psychrophiles: A source of cold-adapted enzymes for energy efficient biotechnological industrial processes [DOI]
- 7 Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya [DOI]
- 8 Protease propeptide structures, mechanisms of activation, and functions [DOI]
- 9 Molecular characterization and growth optimization of halo-tolerant protease producing Bacillus Subtilis Strain BLK-1.5 isolated from salt mines of Karak, Pakistan [DOI]
- 10 Production, Optimization, and Partial Purification of Alkali-Thermotolerant Proteases from Newly Isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12 [DOI]
- 11 Isolation, Screening, and Identification of Alkaline Protease-Producing Bacteria and Application of the Most Potent Enzyme from Bacillus sp. Mar64 [DOI]
- 12 Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya [DOI]
- 13 Detergent-Compatible Proteases: Microbial Production, Properties, and Stain Removal Analysis [DOI]
- 14 Optimization of laccase production using response surface methodology coupled with differential evolution [DOI]
- 15 Characterization of thermostable alkaline proteases from Bacillus infantis SKS1 isolated from garden soil [DOI]
- 16 Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression [DOI]
- 17 Isolation, identification, and whole-genome sequencing of high-yield protease bacteria from Daqu of ZhangGong Laojiu [DOI]
- 18 One Factor at A Time based optimization of protease from poultry associated Bacillus licheniformis [DOI]
- 19 ISOLATION AND IDENTIFICATION OF PROTEASEPRODUCING BACILLUS STRAIN FROM COLD CLIMATE SOIL AND OPTIMIZATION OF ITS PRODUCTION BY APPLYING DIFFERENT FERMENTATION CONDITIONS [DOI]
- 20 SCREENING, OPTIMIZATION OF PRODUCTION AND PARTIAL CHARACTERIZATION OF ALKALINE PROTEASE FROM HALOALKALIPHILIC BACILLUS SP [DOI]
- 21 SCREENING, OPTIMIZATION OF PRODUCTION AND PARTIAL CHARACTERIZATION OF ALKALINE PROTEASE FROM HALOALKALIPHILIC BACILLUS SP
- 22 Growth Media Optimization for Production of Alkaline Protease from Industrial Wastewater using Bacillus subtilis PTCC 1254 [DOI]
- 23 Purification and Characterization of Alkaline Protease from Bacillus sp. HD292 [DOI]
- 24 Production of Microbial Industrial Enzymes [DOI]
- 25 Isolation, production and optimization of endogenous alkaline protease from in-situ sludge and its evaluation as sludge hydrolysis enhancer [DOI]
- 26 Optimization of Protease Production by Bacillus isronensis Strain KD3 Isolaed from Dairy Industry Effluent [DOI]
- 27 Specific Detection of the Gene for the Extracellular Neutral Protease of Bacillus cereus by PCR and Blot Hybridization [DOI]
- 28 Protease production and molecular characterization of a protease dipeptidyl-aminopeptidase gene from different strains of Sordaria fimicola [DOI]
- 29 Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production. [DOI]
- 30 Fast Implementation Of Polynomial-Based Interpolators For Biomedical Image Rotation And Scaling [DOI]
- 31 Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent [DOI]
Cited by 6
Shaimaa M. Azzam, Gad Elsayed Mohamed Salem, Amina A.S. Abdel Rahman · International Journal of Biological Macromolecules · 2026
Musrat Shaheen, Lubna Fazal, Gull Naz · 2025
Ugur Azizoglu, Leandris Argentel‐Martínez, Ofelda Peñuelas‐Rubio · Journal of Basic Microbiology · 2024
Showing 3 of 6 known citations — external sources report more than can currently be individually listed.
Related research
- Screening for Non-communicable Diseases in Public Places in Upper Egypt — shares topic coverage
- Breast Cancer Screening Trend Year 2017 among South-Western Nigeria Female Residents — shares topic coverage
- A Statistical Approach towards Cervical Cancer Awareness among Women in Ghana — shares topic coverage
- Screening for Hypertension and Diabetes in an Underserved Population through Community Outreach; A Case of Rural Community in Enugu State, Nigeria — shares topic coverage
- Comparison of the Diagnostic Yields of Some Methods of Screening for Diabetic Peripheral Neuropathy in North Central Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
27
Page views
0
PDF downloads
3
Outbound clicks
6
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.