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Research Article Open access CC BY 4.0

Molecular Characterization and Optimization of Alkaline Protease Production by Bacillus cereus LS23B

Adefunke Oluwaseun Ogunniran, Adeniyi Success Ohunayo, Iyanuoluwa Oluwaseun Daramola, Titilayo Olufunke Femi-Ola

Asian Journal of Biotechnology and Bioresource Technology · pp. 1–13 · Published 10 Nov 2023

10.9734/ajb2t/2023/v9i4188

Abstract

The study aimed to determine the conditions leading to maximum protease production using submerged fermentation and detecting the presence of protease genes in the bacteria. This is necessary to meet the increasing demand for protease enzymes in the industrial market. The ability of the bacterial isolate to produce protease enzymes was evaluated through primary screening. After that, morphological characterization, biochemical tests, and 16S rRNA analysis were done to identify the bacterial strain. To confirm the presence of the gene-encoding enzyme, the protease (npr) gene primer was amplified using a polymerase chain reaction. The pH, incubation duration, temperature, carbon, and nitrogen sources were studied. Others include metal ions, substrate concentration, and agitation speed. The bacterial strain has 100% similarity to Bacillus cereus A9, while the protease encoding gene was confirmed with positive bands of 951bp. The enzyme was optimally produced at 40 oC with a pH of 9 after 72 h incubation. Starch, gelatin, 1 % substrate concentration, 1mM K+, and agitation speed of 160 rpm fully supported protease production. The presence of the npr gene in the isolate was confirmed. Also, the optimization study reveals that Bacillus cereus LS23B can be used in large-scale protease production, which may be used in different biotechnological applications.

Alkaline protease Bacillus cereus screening protease production culture conditions

References (31)

  1. 1 A review on the application of Bacillus as probiotics in aquaculture [DOI]
  2. 2 Biotechnological Applications of Proteases in Food Technology [DOI]
  3. 3 Proteases: History, discovery, and roles in health and disease [DOI]
  4. 4 Microbial proteases: ubiquitous enzymes with innumerable uses [DOI]
  5. 5 A Review on Microbial Alkaline Proteases
  6. 6 Psychrophiles: A source of cold-adapted enzymes for energy efficient biotechnological industrial processes [DOI]
  7. 7 Molecular Characterization of Low-Density Polyethene (LDPE) Degrading Bacteria and Fungi from Dandora Dumpsite, Nairobi, Kenya [DOI]
  8. 8 Protease propeptide structures, mechanisms of activation, and functions [DOI]
  9. 9 Molecular characterization and growth optimization of halo-tolerant protease producing Bacillus Subtilis Strain BLK-1.5 isolated from salt mines of Karak, Pakistan [DOI]
  10. 10 Production, Optimization, and Partial Purification of Alkali-Thermotolerant Proteases from Newly Isolated Bacillus subtilis S1 and Bacillus amyloliquefaciens KSM12 [DOI]
  11. 11 Isolation, Screening, and Identification of Alkaline Protease-Producing Bacteria and Application of the Most Potent Enzyme from Bacillus sp. Mar64 [DOI]
  12. 12 Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya [DOI]
  13. 13 Detergent-Compatible Proteases: Microbial Production, Properties, and Stain Removal Analysis [DOI]
  14. 14 Optimization of laccase production using response surface methodology coupled with differential evolution [DOI]
  15. 15 Characterization of thermostable alkaline proteases from Bacillus infantis SKS1 isolated from garden soil [DOI]
  16. 16 Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression [DOI]
  17. 17 Isolation, identification, and whole-genome sequencing of high-yield protease bacteria from Daqu of ZhangGong Laojiu [DOI]
  18. 18 One Factor at A Time based optimization of protease from poultry associated Bacillus licheniformis [DOI]
  19. 19 ISOLATION AND IDENTIFICATION OF PROTEASEPRODUCING BACILLUS STRAIN FROM COLD CLIMATE SOIL AND OPTIMIZATION OF ITS PRODUCTION BY APPLYING DIFFERENT FERMENTATION CONDITIONS [DOI]
  20. 20 SCREENING, OPTIMIZATION OF PRODUCTION AND PARTIAL CHARACTERIZATION OF ALKALINE PROTEASE FROM HALOALKALIPHILIC BACILLUS SP [DOI]
  21. 21 SCREENING, OPTIMIZATION OF PRODUCTION AND PARTIAL CHARACTERIZATION OF ALKALINE PROTEASE FROM HALOALKALIPHILIC BACILLUS SP
  22. 22 Growth Media Optimization for Production of Alkaline Protease from Industrial Wastewater using Bacillus subtilis PTCC 1254 [DOI]
  23. 23 Purification and Characterization of Alkaline Protease from Bacillus sp. HD292 [DOI]
  24. 24 Production of Microbial Industrial Enzymes [DOI]
  25. 25 Isolation, production and optimization of endogenous alkaline protease from in-situ sludge and its evaluation as sludge hydrolysis enhancer [DOI]
  26. 26 Optimization of Protease Production by Bacillus isronensis Strain KD3 Isolaed from Dairy Industry Effluent [DOI]
  27. 27 Specific Detection of the Gene for the Extracellular Neutral Protease of Bacillus cereus by PCR and Blot Hybridization [DOI]
  28. 28 Protease production and molecular characterization of a protease dipeptidyl-aminopeptidase gene from different strains of Sordaria fimicola [DOI]
  29. 29 Purification and Characterization of an Extracellular Alkaline Solvent-stable Metalloprotease Secreted from Newly Isolated Bacillus sp. DEM05: Optimization of Protease Production. [DOI]
  30. 30 Fast Implementation Of Polynomial-Based Interpolators For Biomedical Image Rotation And Scaling [DOI]
  31. 31 Isolation, Screening, Characterization and Identification of Alkaline Protease Producing Bacteria from Leather Industry Effluent [DOI]

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