Modulation of Macrophage Activity by a Herbo-mineral Formulation in Murine Model

Abstract

Aim: The present paper investigates the effect on immune responses by a herbo-mineral formulation, Khamira Marwarid [KM], prescribed in the Indian subcontinent as a rejuvenator, especially to convalescing patients of typhoid fever, in murine model. Study Design: KM was administered orally for short, intermediate and long duration [5, 15 and 30 days respectively] at a dose of 2g/kg body weight. Results: Administration of KM enhanced the antigenic and mitogenic activity, induced by ovalbumin and Con A (mitogenic stimuli), of mice whole splenocytes. KM caused a marked increase of production of Th-1 cytokine (IFN-) and a non significant decrease of production of Th-2 cytokine (IL-4) by splenocytes when stimulated with Concanavalin A. Oral administration of KM, by itself did not induce the production of NO by macrophages, but enhanced the production of NO in response to LPS as compared to unstimulated control. However, dose duration related suppression of NO production was observed. KM also enhanced significantly the phagocytosis that was evaluated using the phagocytic rate (PR) and phagocytic index (PI). Conclusion: The results indicate the immunomodulatory potential of KM leading to a Th1 dominant immune state and activation of macrophages and may find use in immunotherapy of tumors.