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Research Article Open access CC BY 4.0

Microencapsulation: Toward the Reduction of the Salinity Stress Effect on Wheat Plants Using NPK Rhizobacteria

Maged M. Saad, Hanaa A. Abo-Koura, Mounira M. Bishara, Ibrahim M. Gomaa

Biotechnology Journal International · pp. 1–18 · Published 24 Jan 2020

10.9734/bji/2019/v23i430091

Abstract

Salinity is one of the most vicious environmental factors controlling the productivity of crop plants as most of the crop plants are sensitive to salinity affected by high concentrations of salts in the soil. The objective of this study was to evaluate the influence of the inoculation with the encapsulated and liquid culture of three halo-tolerant plants growth-promoting rhizobacteria (PGPR) strains of Paenibacillus polymyxa MSRH5, Bacillus nakamurai MSRH1 and Bacillus pacificus MSR H3 on the growth and yield of wheat (Triticum aestivum L.). The three strains MSRH1, MSRH3 and MSR H5 were characterized as salt-tolerant bacteria. P. polymyxa MSRH5 had nitrogen fixation ability while B. nakamurai MSRH1 and B. pacificus MSRH3 were able to solubilize phosphate and K respectively. All strains can produce indole acidic acid (IAA) and exopolysaccharides (EPS) under saline conditions. Encapsulated beads were observed under Scanning Electron Microscope (SEM). Colonization of encapsulated bacteria on the root of the wheat plant was studied by Transmission Electron Microscopy (TEM). Under soil salinity conditions in two consecutive field tries, results cleared that strains in two forms succeed to colonize the plant root, the reduction in shoot proline was 35.8% with capsules inoculation as well as improved relative water content (%) to 60.57% and improved the electrolyte leakage recorded 18.1% respectively compared to control. Generally, halo-tolerant PGPR inoculation increased DHA (?), acidic and alkaline phosphatase activities compared to control, inoculation with capsules exhibited a reduction in catalase enzymes 46.00%, 37.5% in ascorbate peroxidase and 40% in superoxide dismutase respectively in shoots of the wheat plant. There is a significant increase in all yield parameters, the highest plant height 115.8 cm, spike length 21 cm and 1000 grains 71.3 g respectively recorded with capsules inoculation, it had considerable effects on the content of N, P, K and Na in shoots of wheat plants and reduced the value of Na/K ratio in all treatments inoculated compared to un-inoculated wheat plant.

Halo-tolerant bacteria PGPR alginate beads wheat colonization.

References (40)

  1. 1 Rapid determination of free proline for water-stress studies [DOI]
  2. 2 Mechanisms of Salinity Tolerance [DOI]
  3. 3 Soil and Plant Analysis [DOI]
  4. 4 Methods of Soil Analysis 2d ed., pt. 1; Physical and Mineralogical Methods [DOI]
  5. 5 Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation [DOI]
  6. 6 A critical examination of the specificity of the salkowski reagent for indolic compounds produced by phytopathogenic bacteria [DOI]
  7. 7 Drought and salinity: A comparison of their effects on mineral nutrition of plants [DOI]
  8. 8 Efficient soil microorganisms: A new dimension for sustainable agriculture and environmental development [DOI]
  9. 9 Inoculation with selected strains of Azospirillum brasilense and A. lipoferum improves yields of maize and wheat in Brazil [DOI]
  10. 10 Plant growth promoting rhizobacteria Dietzia natronolimnaea modulates the expression of stress responsive genes providing protection of wheat from salinity stress [DOI]
  11. 11 Plant Growth-Promoting Rhizobacteria Enhance Abiotic Stress Tolerance in Solanum tuberosum Through Inducing Changes in the Expression of ROS-Scavenging Enzymes and Improved Photosynthetic Performance [DOI]
  12. 12 Transient salinity and subsoil constraints to dryland farming in Australian sodic soils: an overview [DOI]
  13. 13 Bacterial solubilization of mineral phosphates: Historical perspective and future prospects [DOI]
  14. 14 Biofortification of wheat through inoculation of plant growth promoting rhizobacteria and cyanobacteria [DOI]
  15. 15 Microencapsulation of microbial cells [DOI]
  16. 16 Bacterial exopolysaccharide and biofilm formation stimulate chickpea growth and soil aggregation under salt stress [DOI]
  17. 17 Effects of Different Treatments of Salicylic Acid on Heat Tolerance, Chlorophyll Fluorescence, and Antioxidant Enzyme Activity in Seedlings of Cucumis sativa L. [DOI]
  18. 18 OSMOADAPTATION BY RHIZOSPHERE BACTERIA [DOI]
  19. 19 Mechanisms of salt tolerance and interactive effects of Azospirillum brasilense inoculation on maize cultivars grown under salt stress conditions [DOI]
  20. 20 Inoculation of Brassica oxyrrhina with plant growth promoting bacteria for the improvement of heavy metal phytoremediation under drought conditions [DOI]
  21. 21 Plant Tissue Culture: Techniques and Experiments
  22. 22 Endophytic bacteria (Sphingomonassp. LK11) and gibberellin can improveSolanum lycopersicumgrowth and oxidative stress under salinity [DOI]
  23. 23 Prescott's Principles of Microbiology
  24. 24 Paddy plants inoculated with PGPR show better growth physiology and nutrient content under saline conditions [DOI]
  25. 25 Effect of immobilized phosphate solubilizing bacteria on wheat growth and phosphate uptake [DOI]
  26. 26 Pseudomonas fluorescens LBUM223 Increases Potato Yield and Reduces Common Scab Symptoms in the Field [DOI]
  27. 27 Viability evaluation of alginate-encapsulated Pseudomonas putida Rs-198 under simulated salt-stress conditions and its effect on cotton growth [DOI]
  28. 28 Colonization of Potato Rhizosphere by GFP-Tagged Bacillus subtilis MB73/2, Pseudomonas sp. P482 and Ochrobactrum sp. A44 Shown on Large Sections of Roots Using Enrichment Sample Preparation and Confocal Laser Scanning Microscopy [DOI]
  29. 29 The Effect of Cell Immobilization by Calcium Alginate on Bacterially Induced Calcium Carbonate Precipitation [DOI]
  30. 30 Wheat response to interactive effects of boron and salinity [DOI]
  31. 31 Effect of encapsulated cells of Enterobacter sp on plant growth and phosphate uptake [DOI]
  32. 32 Biodiversity and phylogenetic analysis of culturable bacteria indigenous to Khewra salt mine of Pakistan and their industrial importance [DOI]
  33. 33 Global Impacts of European Agricultural and Biofuel Policies [DOI]
  34. 34 The efficiency of Plant Growth Promoting Rhizobacteria (PGPR) on yield and yield components of two varieties of wheat in salinity condition.
  35. 35 DYNAMICS OF FABA BEAN GROWTH AND NUTRIENT UPTAKE AND THEIR CORRELATION WITH GRAIN YIELD [DOI]
  36. 36 Encapsulation of plant growth promoting inoculant in bacterial alginate beads enriched with humic acid
  37. 37 PHYSIOLOGICAL DIFFERENCES BETWEEN EFFECTIVE AND INEFFECTIVE STRAINS OF RHIZOBIUM [DOI]
  38. 38 Phosphate-solubilizing microbes in rhizosphere soils of 19 weeds in southeastern China [DOI]
  39. 39 ALGINATE BASED MACROCAPSULES AS INOCULANTS CARRIERS FOR PRODUCTION OF NITROGEN BIOFERTILIZERS
  40. 40 Supplementary Potassium Nitrate Improves Salt Tolerance in Bell Pepper Plants [DOI]

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