Low Cost Micropropagation of Local Varieties of Taro (Colocasia esculenta spp.)
Alex Ngetich, Steven Runo, Omwoyo Ombori, Michael Ngugi, Fanuel Kawaka, Arusei Perpetua, Gitonga Nkanata
Biotechnology Journal International · pp. 136–145 · Published 23 Feb 2015
10.9734/BBJ/2015/15614Abstract
Aims: This study was conducted to evaluate low cost protocol for the micropropagation of three varieties of taro (Dasheen, Eddoe and wild) from eastern Kenya. Study Design: The plants were grown in polythene bags arranged in a completely randomized block design (CRBD) replicated nine times. Place and Duration of Study: Department of plant sciences Kenyatta University in plant and tissue culture laboratory, between June 2010 and December 2011. Methodology: The three media types tested were Omex foliar feed (LCM1), Stanes micronutrients (LCM2) and micro food (LCM3) as substitute for Murashige and Skoog (MS) media. Results: The results showed significant differences (p˂0.05) in the shoot generation for Eddoe and wild varieties in LCM1 and LCM2 respectively compared to LCM3 and MS. Plants grown in MS media and LCM3 had the longest height compared to LCM1 and LCM2. Naphthalene Acetic Acid (NAA) and Citishooter did not show any significant differences on the number of roots. All the regenerated plants in this study were similar in morphology and vigour. Media cost was reduced by 94.7% (LCM1) and 96% for both LCM2 and LCM3. Conclusion: This study indicates the potential of low cost media as a substitute for conventional micro propagation.
References (8)
- 1 Plant biotechnology: a tool for development in Africa [DOI]
- 2 Incidence and distribution of viruses of Taro (Colocasia esculenta) in Pacific Island countries [DOI]
- 3 A simple and low-cost strategy for micropropagation of cassava (Manihot esculenta Crantz). [DOI]
- 4 Table sugar as an alternative low cost medium component for in vitro micro-propagation of potato (Solanum tuberosum L.) [DOI]
- 5 Low technology tissue culture materials for initiation and multiplication of banana plants [DOI]
- 6 Cost-effective nutrient sources for tissue culture of cassava (Manihot esculenta Crantz) [DOI]
- 7 In vitro micropropagation of white dasheen ( Colocassia esculenta ) [DOI]
- 8 A Low-cost Medium for Sweet Potato Micropropagation
Cited by 16
Joyce Wambui Njuguna, Anne N. Karuma, Patrick Gicheru · Tropical and Subtropical Agroecosystems · 2023
Sinkiyian Kepue Mercy, M. Mweu Cecilia, Muruu Kariuki David · African Journal of Biotechnology · 2021
Vincent Lebot, Anton Ivančič · Euphytica · 2022
Anne N Karuma, Joyce Wambui Njuguna, Patrick Gicheru · Tropical and Subtropical Agroecosystems · 2024
Lorena Perea-Gómez, José Alberto García-Salazar, María del Carmen López-Reyna · Madera y Bosques · 2022
G. T. Kujeke, T. C. Chitendera, R. T. Masekesa · Advances in Agriculture · 2020
Bogale Adelegn · Journal of Agricultural Biotechnology and Sustainable Development · 2018
Joyce Wambui Njuguna, Anne N. Karuma, Patrick Gicheru · International Journal of Agronomy · 2023
Showing 8 of 16 known citations — external sources report more than can currently be individually listed.
Related research
- Evaluation of Serum Copper and Zinc Concentrations in Relation to Clinical Status among Adult People Living with HIV/AIDS in North-West Ethiopia — shares topic coverage
- Quality Evaluation of Ready-To-Eat Garri Made from Cassava Mash and Mango Fruit Mesocarp Blends — shares topic coverage
- Response of Pigeonpea (Cajanus cajan L.) to Seed Polymerization with Micronutrients and Foliar Spray at Different Growth Stages — shares topic coverage
- Antinutrients and Bioavailability of Nutrients in Maize, Cassava and Soybeans Composite Flour — shares topic coverage
- Assessment of Micronutrients of Solanum aethiopicum and Solanum melongena Fruits Consumed in Burkina Faso — shares topic coverage
Article metrics
Real usage data collected on this platform.
3
Page views
0
PDF downloads
0
Outbound clicks
16
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.