LC-MS, GC-MS and Hematological Profile of  Mucuna pruriens Extracts in Alloxan Induced Diabetic Albino Rat

E. C. Chukwu; K. U. Osuocha; B. Musa; J. C. Njoku

doi:10.9734/ijbcrr/2022/v31i130300

Research Article Open access CC BY 4.0

LC-MS, GC-MS and Hematological Profile of Mucuna pruriens Extracts in Alloxan Induced Diabetic Albino Rat

E. C. Chukwu, K. U. Osuocha, B. Musa, J. C. Njoku

International Journal of Biochemistry Research & Review · pp. 54–64 · Published 28 Feb 2022

10.9734/ijbcrr/2022/v31i130300

Abstract

Mucuna pruriens also known as velvet beans is an annual climbing shrub that has been claimed traditionally to possess anti anemic potentials. The present study evaluated bioactive constituents and hematological profile of Mucuna pruriens seed and leaf extracts on alloxan induced diabetic male albino rat using standard analytical method. Result of LC-MS investigation on the seed shows the presence of Hydroxystreptozolin ( Oxazolidene) and Dihydrocapsaicin (Meltoxyphe.nol) while the leaf reveals the presence of four(4) bioactive compound: Furocoumurine acid, Epioxylubianin, Acetyllaustoinid and Quassin. The GC-MS studies on Mucuna pruriens seed indicates the presence of twelve bioactive compound. 2-methyl-z,z-3,13-octadecadienol cis-13-octadecadienoic acid, methylester hexadecanoic acid, methylester pentadecanoic acid, 9,12,octadecadienoic acid (z, z)-methylester, 11-octadenoic acid, methylester q-octadecenoic acid, Octadecanamide- N-(2-hydroxyethyl) dodecanamide,N-(2-hydroxyethyl), urea,triethyl-Ethanamine, N, N-dimethyl (phenylmethoxy), 13-Hexyloxacyclotridec carbonitrile,3,5bis[C2dimethylamino]ethylthio, 12-octadecadienoic acid(z, z)-cis-13-octadecenoic acid, 9,12-octadecadienoicacid(z,z)-2-hydroxy-1(Hydroxymethyl) ethylester, cyclopentadecanane,2-hydroxy-9,12-octadeadienol,LE, etc while GC-MS analysis of the leaf reveals the presence of twenty bioactive compounds such as Nonyl alcohol, 3-Undecene, 1-Dodecene etc. Findings from this study indicates significant improvement on the hematological indices in treated groups relative to untreated. The restorative hematological efficacy of Mucuna pruriens extract is indicative of its traditional relevance in medicinal and therapeutic applications and hence may justify its local claims as having anti-anemic functionality.

Mucuna pruriens hematological profile alloxan

References (9)

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Hepatic, Pancreatic, and Renal Histo-Morphologic Alterations in Administration of Aqueous and Ethanol Seed Extract of Buchholzia coriacea in Alloxan-Induced Diabetic Rats — shares topic coverage
Effect of Aqueous Extract of Persea americana Seed on Blood Glucose in Alloxan-induced Diabetic Wistar Rats — shares topic coverage
Comparative Effects of Fermented and Unfermented Parkia biglobosa Bean Seeds in Diabetic Induced Experimental Rats — shares topic coverage
Effect of Composite Flours of Finger Millet, Bambara Groundnut and ‘Khain’ (Lecaniodiscus Cupanioides) on Blood Glucose Response, Lipids, and Liver Enzymes of Alloxan-Induced Diabetic Rats — shares topic coverage
Comparison of Antidiabetic Effect of Ethanolic Leaves Extract of Mangifera indica and Moringa oleifera on Alloxan Induced Diabetic Rats — shares topic coverage

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E. C. Chukwu, K. U. Osuocha, B. Musa, J. C. Njoku (2022). LC-MS, GC-MS and Hematological Profile of Mucuna pruriens Extracts in Alloxan Induced Diabetic Albino Rat. International Journal of Biochemistry Research & Review , 54–64. https://doi.org/10.9734/ijbcrr/2022/v31i130300

Affiliations

E. C. Chukwu — Department of Biochemistry, Federal University of Lafia, Nasarawa State, Nigeria.
K. U. Osuocha — Biochemistry Unit, Department of Science Laboratory Technology, Federal University of Lafia, Nasarawa State, Nigeria.
B. Musa — Centre for Dry Land Agriculture, Bayero University Kano, Nigeria.
J. C. Njoku — Department of Pharmacology, Niger Delta University , Bayelsa State, Nigeria.

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